Volume 36 Issue 5
Sep.  2015
Turn off MathJax
Article Contents

Tian-Zi WANG, Ai SUN, Na WANG, Zhong-Kai CUI, Song-Lin CHEN, Zhen-Xia SHA. Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis). Zoological Research, 2015, 36(5): 305-310. doi: 10.13918/j.issn.2095-8137.2015.5.305
Citation: Tian-Zi WANG, Ai SUN, Na WANG, Zhong-Kai CUI, Song-Lin CHEN, Zhen-Xia SHA. Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis). Zoological Research, 2015, 36(5): 305-310. doi: 10.13918/j.issn.2095-8137.2015.5.305

Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis)

doi: 10.13918/j.issn.2095-8137.2015.5.305
Funds:  This study was supported by grants from the National Natural Science Foundation of China (31130057), the State 863 High-Technology R&D Project of China (2012AA10A408), the Taishan Scholar Project Fund of Shandong Province of China and the Special Scientific Research Funds for the Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2013A0402)
  • Received Date: 2015-03-18
  • Publish Date: 2015-09-18
  • An astroglial cell line was established from the brain of half smooth tongue sole (Cynoglossus semilaevis) and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein (GFAP), which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2-Mercaptoethanol (2-Me) and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number (2n=42). The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.
  • 加载中
  • [1] Clem LW, Ely IE, Wilson M, Chinchar VG, Stuge T, Barker K, Luft C, Rycyzyn M, Hogan RJ, van Lopik T, Miller NW. 1996. Fish immunology: the utility of immortalized lymphoid cells-a mini review. Veterinary Immunology and Immunopathology , 54(1-4): 137-144.
    [2] Dahl D, Bignami A. 1973. Immunochemical and immunofluorescence studies of the glial fibrillary acidic protein in vertebrates. Brain Research , 61: 279-93.
    [3] Fan LC, Collodi P. 2002. Progress towards cell-mediated gene transfer in zebrafish. Briefings in functional genomics & proteomics , 1(2): 131-138.
    [4] Fan TJ, Geng XF, Cong RS, Jiang GJ, Yu QT, Fu YF, Wang J, Yu MM, Yang XX, Wu JD. 2007. Establishment of a novel fin cell line from Turbot, Scophthalmus maximus . Periodical of Ocean University of China , 2007(5): 759-766.
    [5] Freshney RI. 1994. Culture of animal cells: A manual of basic techniques. New York: Wiley-Liss Inc., 387-389.
    [6] Frjd EM, Westerlund J, Isomaa B. 2002. Culturing and characterization of astrocytes isolated from juvenile rainbow trout ( Oncorhynchus mykiss ). Comparative Biochemistry and Physiology A: Molecular &Integrative Physiology , 133(1): 17-28.
    [7] Grupp L, Wolburg H, Mack AF. 2010. Astroglial structures in the zebrafish brain. Journal of Comparative Neurology , 518(21): 4277-4278.
    [8] Kalman M. 1998. Astroglial architecture of the carp ( Cyprinus carpio ) brain as revealed by immunohistochemical staining against glial fibrillary acidic protein (GFAP). Anatomy and Embryology (Berlin) , 198(5): 409-433.
    [9] Kalman M. 2002. GFAP expression withdraws-a trend of glial evolution?. Brain Research Bulletin , 57(3-4): 509-511.
    [10] Kengaku M, Okamoto H. 1995. bFGF as a possible morphogen for the anteroposterior axis of the central nervous system in Xenopus . Development , 121(9): 3121-3130.
    [11] Kraft AW, Hu XY, Yoon H, Yan P, Xiao QL, Wang Y, Gil SC, Brown J, Wilhelmsson U, Restivo JL, Cirrito JR, Holtzman DM, Kim J, Pekny M, Lee JM. 2013. Attenuating astrocyte activation accelerates plaque pathogenesis in APP/PS1 mice. The Journal of Federation of American Societies for Experimental Biology , 27(1): 187-198.
    [12] Levan A, Fredga K, Sandberg AA. 1964. Nomenclature for centromeric position on chromosomes. Hereditas , 52: 201-220.
    [13] Lu YA, Aguirre AA, Wang Y, Zeng LB, Loh PC, Yanagihara R. 2004. Viral susceptibility of newly established cell lines from the Hawaiian monk seal Monachus schauinslandi. Diseases of Aquatic Organisms , 57(3): 183-191.
    [14] Mack AF, Tiedemann K. 2013. Cultures of astroglial cells derived from brain of adult cichlid fish. Journal of Neuroscience Methods , 212(2): 269-275.
    [15] Oh YB, Lee JS, Park EH. 2001. Fish cell line as an in vitro test system for analyzing chromosome aberrations. Bulletin of Environmental Contamination and Toxicology , 67(1): 6-11.
    [16] Rodea M, Berga T, Gjøena T. 1997. Effect of temperature on endocytosis and intracellular transport in the cell line SHK-1 derived from salmon head kidney. Comparative Biochemistry and Physiology Part A : Physiology , 117(4): 531-537.
    [17] Ruiz S, Schyth BD, Encinas P, Tafalla C, Estepa A, Lorenzen N, Coll JM. 2009. New tools to study RNA interference to fish virus: fish cell lines permanently expressing siRNAs targeting the viral polymerase of viral hemorrhagic septicemia virus. Antiviral Research , 82(3): 148-156.
    [18] Salinas I, Meseguer J, Esteban MA. 2008. Antiproliferative effects and apoptosis induction by probiotic cytoplasmic extracts in fish cell lines. Veterinary Microbiology , 126(1-3): 287-294.
    [19] Schwartz M, Belkin M, Harel A, Lavie V, Hadani M, Rachailovich I, Stein-Izsak C. 1985. Regenerating fish optic nerves and a regeneration-like response in injured optic nerves of adult rabbits. Science , 228(4699): 600-603.
    [20] Shao W, Zhang SZ, Tang M, Zhang XH, Zhou Z, Yin YQ, Zhou QB, Huang YY, Liu YJ, Wawrousek E, Chen T, Li SB, Xu M, Zhou JN, Hu G, Zhou JW. 2012. Suppression of neuroinflammation by astrocytic dopamine D2 receptors via αB-crystallin. Nature , 494(7435): 90-94.
    [21] Wang GT, LaPatra S, Zeng LB, Zhao ZS, Lu YA. 2004. Establishment, growth, cryopreservation and species of origin identification of three cell lines from white sturgeon, Acipenser transmontanus. Methods in Cell Science , 25(3-4): 211-220.
    [22] Wang XL, Wang N, Sha ZX, Chen SL. 2010. Establishment, characterization of a new cell line from heart of half smooth tongue sole ( Cynoglossus semilaevis ). Fish Physiology and Biochemistry , 36(4): 1181-1189.
    [23] Wei YB, Fan TJ, Jiang GJ, Sun A, Xu XH, Wang J. 2009. Establishment of a novel fin cell line from Brown-marbled grouper, Epinephelus fuscoguttatus (Forsskål), and evaluation of its viral susceptibility. Aquaculture Research , 40(13): 1523-1531.
    [24] Wen CM, Huang JY, Ciou JH, Kao YL, Cheng YH. 2009. Immunochemical and molecular characterization of GBC4 as a tanycyte-like cell line derived from grouper brain. Comparative Biochemistry and Physiology A: Molecular &Integrative Physiology , 153(2): 191-201.
    [25] Wen CM, Lee CW, Wang CS, Cheng YH, Huang HY. 2008. Development of two cell lines from Epinephelus coioides brain tissue for characterization of betanodavirus and megalocytivirus infectivity and propagation. Aquaculture , 278: 14-21.
    [26] Wen CM, Wang CS, Chin TC, Cheng ST, Nan FH. 2010. Immunochemical and molecular characterization of a novel cell line derived from the brain of Trachinotus blochii (Teleostei, Perciformes): A fish cell line with oligodendrocyte progenitor cell and tanycyte characteristics. Comparative Biochemistry and Physiology A: Molecular &Integrative Physiology , 156(2): 224-231.
    [27] Yiu G, He Z. 2006. Glial inhibition of CNS axon regeneration. Nature Review of Neuroscience , 7(8): 617-627.
    [28] Zhang B, Wang XL, Sha ZX, Yang CG, Liu SS, Wang N, Chen SL. 2011. Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis . International Journal of Biological Sciences , 7(4): 452-459.
    [29] Zheng Y, Wang N, Xie MS, Sha ZX, Chen SL. 2012. Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole ( Cynoglossus semilaevis ). Fish Physiology and Biochemistry , 38(6): 1635-1643.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(410) PDF downloads(1029) Cited by()

Related
Proportional views

Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis)

doi: 10.13918/j.issn.2095-8137.2015.5.305
Funds:  This study was supported by grants from the National Natural Science Foundation of China (31130057), the State 863 High-Technology R&D Project of China (2012AA10A408), the Taishan Scholar Project Fund of Shandong Province of China and the Special Scientific Research Funds for the Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2013A0402)

Abstract: An astroglial cell line was established from the brain of half smooth tongue sole (Cynoglossus semilaevis) and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein (GFAP), which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2-Mercaptoethanol (2-Me) and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number (2n=42). The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.

Tian-Zi WANG, Ai SUN, Na WANG, Zhong-Kai CUI, Song-Lin CHEN, Zhen-Xia SHA. Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis). Zoological Research, 2015, 36(5): 305-310. doi: 10.13918/j.issn.2095-8137.2015.5.305
Citation: Tian-Zi WANG, Ai SUN, Na WANG, Zhong-Kai CUI, Song-Lin CHEN, Zhen-Xia SHA. Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis). Zoological Research, 2015, 36(5): 305-310. doi: 10.13918/j.issn.2095-8137.2015.5.305
Reference (29)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return