Volume 36 Issue 1
Jan.  2015
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David M. IRWIN. Genomic organization and evolution of ruminant lysozyme c genes. Zoological Research, 2015, 36(1): 1-17. doi: 10.13918/j.issn.2095-8137.2015.1.1
Citation: David M. IRWIN. Genomic organization and evolution of ruminant lysozyme c genes. Zoological Research, 2015, 36(1): 1-17. doi: 10.13918/j.issn.2095-8137.2015.1.1

Genomic organization and evolution of ruminant lysozyme c genes

doi: 10.13918/j.issn.2095-8137.2015.1.1
Funds:  This study was supported by grants from the Natural Sciences and Engineering Research Council (RGPIN 183701)
Supplemental Figure 1. Predicted lysozyme c cDNA sequences in fasta format
More Information
  • Corresponding author: David M. IRWIN
  • Received Date: 2014-10-08
  • Rev Recd Date: 2014-12-02
  • Publish Date: 2015-01-08
  • Ruminant stomach lysozyme is a long established model of adaptive gene evolution. Evolution of stomach lysozyme function required changes in the site of expression of the lysozyme c gene and changes in the enzymatic properties of the enzyme. In ruminant mammals, these changes were associated with a change in the size of the lysozyme c gene family. The recent release of near complete genome sequences from several ruminant species allows a more complete examination of the evolution and diversification of the lysozyme c gene family. Here we characterize the size of the lysozyme c gene family in extant ruminants and demonstrate that their pecoran ruminant ancestor had a family of at least 10 lysozyme c genes, which included at least two pseudogenes. Evolutionary analysis of the ruminant lysozyme c gene sequences demonstrate that each of the four exons of the lysozyme c gene has a unique evolutionary history, indicating that they participated independently in concerted evolution. These analyses also show that episodic changes in the evolutionary constraints on the protein sequences occurred, with lysozyme c genes expressed in the abomasum of the stomach of extant ruminant species showing the greatest levels of selective constraints.
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