Volume 35 Issue 6
Nov.  2014
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Ya HE, Zheng-Huan WANG, Xiao-Ming WANG. Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation. Zoological Research, 2014, 35(6): 528-536. doi: 10.13918/j.issn.2095-8137.2014.6.528
Citation: Ya HE, Zheng-Huan WANG, Xiao-Ming WANG. Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation. Zoological Research, 2014, 35(6): 528-536. doi: 10.13918/j.issn.2095-8137.2014.6.528

Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation

doi: 10.13918/j.issn.2095-8137.2014.6.528
Funds:  The work was supported by the National Natural Science Foundation of China (30870308 and 31071944) and the Shanghai Rising-Star Program (10QA1402200)
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  • Corresponding author: Xiao-Ming WANG
  • Received Date: 2014-03-25
  • Rev Recd Date: 2014-08-01
  • Publish Date: 2014-11-08
  • Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.
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