Volume 35 Issue 6
Nov.  2014
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Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492
Citation: Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492

Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

doi: 10.13918/j.issn.2095-8137.2014.6.492
Funds:  This study was supported by the National 863 Project of China ( 2012AA021801) and the Project of Frontier Study of Foundation, CAS (KSCX2-EW-R-11, KSCX2-EW-J-23)
More Information
  • Corresponding author: Ren LAI
  • Received Date: 2014-03-23
  • Rev Recd Date: 2014-06-25
  • Publish Date: 2014-11-08
  • Endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews (Tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis (detection rate 42%), Pseudomonas aeruginosa (25%), Staphlococcus aureus (33%), S. Epidermidis (75%), Micrococcus luteus (25%), Kurthia gibsonii (17%); oral cavity: Neisseria mucosa (58%), Streptococcus pneumonia (17%); anus: Enterococcus faecalis (17%), Lactococus lactis (33%), Escherichia coli (92%), Salmonella typhosa (17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis (75%); oral cavity: N.mucosa (67%); anus: L. lactis (33%), E. coli (100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.
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  • [1] Dong L, Lü LB, Lai R. 2011. Molecular cloning of Tupaia belangeri chinensis neuropeptide Y and homology comparison with other analogues from primates. Zoological Research, 33(1): 75-78.
    [2] Gao JH, Jang QF, Luo ZW, Sun XM, Dai JJ. 2009. Culture, isolation and identification of normal intestinal bacterial flora and their antibiotic susceptibility in tree shrew. Chinese Journal of Comparative Medicine, 19(12): 24-26.
    [3] He B, Han D, He L, Zhang FY, Li SQ. 2012. The research of magnetic resonance imaging of thrombotic cerebral ischemia in tree shrews. Journal of Clinical Radiology, 31(10): 1492-1496.
    [4] Huang XY, Xu J, Sun XM, Dai JJ. 2013. Development of application of tree shrew in human disease animal models research. Laboratory Animal Science, 30(2): 59-64.
    [5] Jiang QF, Kuang DX, Tong PF, Sun XM, Dai JJ. 2011. Scale breeding of tree shrews and the establishment of breeding population. Laboratory Animal Science, 28(6): 35-38.
    [6] Katznelson H, Gillespie DC, Cook FD. 1964. Studies on the relationships between nematodes and other soil microorganisms. 3. Lytic action of soil myxobacters on certain species of nematodes. Canadian Journal of Microbiology, 10, 699-704.
    [7] Li G, Yan Y, Chang YY, Lü LB. 2009. New method of the tree shrews (Tupaia belangeri chinensis) breeding in laboratory. Husbandry and Veterinary of Modern, (3): 18-21.
    [8] Li Y, Dai JJ, Sun XM, Xia XS. 2011. Progress in studies on HCV receptor of Tupaia as a potential hepatitis C animal model. Zoological Research, 32(1): 97-103.
    [9] Shen PQ, Zheng H, Liu RW, Chen LL, Li B, He BL, Li JT, Ben KL, Cao YM, Jiao JL. 2011. Progress and prospect in research on laboratory tree shrew in China. Zoological Research, 32(1): 109-114.
    [10] Simpson GG. 1945. The principles of classification and a classification of mammals. Bull Amer Mus Nat Hist, 95: 1-22.
    [11] Wang J, Zhou QX, Lü LB, Xu L, Yang YX. 2012a. A depression model of social defeat etiology using tree shrews. Zoological Research, 33(1): 92-98.
    [12] Wang WG, Huang XY, Xu J, Sun XM, Dai JJ, Li QH. 2012b. Experimental studies on infant Tupaia belangeri chineses with EV71 infection. Zoological Research, 33(1): 7-13.
    [13] Wang XJ, Yang C, Su JJ. 2010. Development of application of tree shrew in experimental medical research. Chinese Journal of Comparative Medicine, 20(2): 67-70.
    [14] Wang XX, Li JX, Wang WG, Sun XM, He CY, Dai JJ. 2011. Preliminary investigation of viruses to the wild tree shrews (Tupaia belangeri chinese). Zoological Research, 32(1): 66-69.
    [15] Wang YC, Jin HX, Tang YM, Liao GY, Xu JY. 1987. Analysis of intestinal pathogen for the healthy tree shrews. Medical Biology Research, 1: l4.
    [16] Wu XY, Li YH, Chang Q, Zhang LQ, Liao SS, Liang B. 2013. Streptozotocin induction of type 2 diabetes in tree shrew. Zoological Research, 34(2): 108-115.
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Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews

doi: 10.13918/j.issn.2095-8137.2014.6.492
Funds:  This study was supported by the National 863 Project of China ( 2012AA021801) and the Project of Frontier Study of Foundation, CAS (KSCX2-EW-R-11, KSCX2-EW-J-23)
    Corresponding author: Ren LAI

Abstract: Endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews (Tupaia belangeri chinensis) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis (detection rate 42%), Pseudomonas aeruginosa (25%), Staphlococcus aureus (33%), S. Epidermidis (75%), Micrococcus luteus (25%), Kurthia gibsonii (17%); oral cavity: Neisseria mucosa (58%), Streptococcus pneumonia (17%); anus: Enterococcus faecalis (17%), Lactococus lactis (33%), Escherichia coli (92%), Salmonella typhosa (17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis (75%); oral cavity: N.mucosa (67%); anus: L. lactis (33%), E. coli (100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.

Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492
Citation: Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499. doi: 10.13918/j.issn.2095-8137.2014.6.492
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