Volume 35 Issue 5
Sep.  2014
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Yu-Cheng JI, Hui ZHUGE, Shan-Shan ZHANG, Shu-Fang ZHANG, Xian-Yu Yang. Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis. Zoological Research, 2014, 35(5): 398-403. doi: 10.13918/j.issn.2095-8137.2014.5.398
Citation: Yu-Cheng JI, Hui ZHUGE, Shan-Shan ZHANG, Shu-Fang ZHANG, Xian-Yu Yang. Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis. Zoological Research, 2014, 35(5): 398-403. doi: 10.13918/j.issn.2095-8137.2014.5.398

Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis

doi: 10.13918/j.issn.2095-8137.2014.5.398
Funds:  This study was supported by the National Natural Science Foundation of China (31071181, 31372149) and the Students' Innovative Training Program of ZAFU (20120207, 20120213)
More Information
  • Corresponding author: Xian-Yu Yang
  • Received Date: 2014-02-17
  • Rev Recd Date: 2014-03-25
  • Publish Date: 2014-09-08
  • In this study, to clarify the bioactive polypeptides included in the skins and secretions of Bufo, we screened the Japanese toad (Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction (PCR), and obtained a transcript of 1 075 bp consisting of 1 37 bp 5' untranslated region (UTR), 515 bp 3' UTR and a 423 bp open reading frame (ORF) encoding a polypeptide of 140 amino acid residues (GenBank accession number: KF359945). Homolog analysis showed a 70%-96% homology with sterol carrier protein-2 (SCP-2) present in other animals, which is implicated in lipid metabolism of other organisms. The gene SCP-2 of Chinese toad (B. gargarizans) was cloned from a first strand cDNA of Bufo skin (GenBank accession number: KF381341) via PCR, whose encoding polypeptide has only one amino acid difference from that of Japanese toad. Tissue distribution analysis showed that SCP-2 expressed in all organs tested, though in the liver and spleen it manifested lower expression than in other organs. These findings might indicate SCP-2 being one of the active ingredients in toad skin. These findings may in turn have implications for further drug development from traditional Chinese medicine sources.
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Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis

doi: 10.13918/j.issn.2095-8137.2014.5.398
Funds:  This study was supported by the National Natural Science Foundation of China (31071181, 31372149) and the Students' Innovative Training Program of ZAFU (20120207, 20120213)
    Corresponding author: Xian-Yu Yang

Abstract: In this study, to clarify the bioactive polypeptides included in the skins and secretions of Bufo, we screened the Japanese toad (Bufo japonicus formosus) skin cDNA library by colony polymerase chain reaction (PCR), and obtained a transcript of 1 075 bp consisting of 1 37 bp 5' untranslated region (UTR), 515 bp 3' UTR and a 423 bp open reading frame (ORF) encoding a polypeptide of 140 amino acid residues (GenBank accession number: KF359945). Homolog analysis showed a 70%-96% homology with sterol carrier protein-2 (SCP-2) present in other animals, which is implicated in lipid metabolism of other organisms. The gene SCP-2 of Chinese toad (B. gargarizans) was cloned from a first strand cDNA of Bufo skin (GenBank accession number: KF381341) via PCR, whose encoding polypeptide has only one amino acid difference from that of Japanese toad. Tissue distribution analysis showed that SCP-2 expressed in all organs tested, though in the liver and spleen it manifested lower expression than in other organs. These findings might indicate SCP-2 being one of the active ingredients in toad skin. These findings may in turn have implications for further drug development from traditional Chinese medicine sources.

Yu-Cheng JI, Hui ZHUGE, Shan-Shan ZHANG, Shu-Fang ZHANG, Xian-Yu Yang. Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis. Zoological Research, 2014, 35(5): 398-403. doi: 10.13918/j.issn.2095-8137.2014.5.398
Citation: Yu-Cheng JI, Hui ZHUGE, Shan-Shan ZHANG, Shu-Fang ZHANG, Xian-Yu Yang. Cloning the sterol carrier protein 2 genes of Japanese toad (Bufo japonicus formosus) and Chinese toad (Bufo gargarizans) and its tissue expression analysis. Zoological Research, 2014, 35(5): 398-403. doi: 10.13918/j.issn.2095-8137.2014.5.398
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