Volume 35 Issue 5
Sep.  2014
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Qiao-Qing XU, Dai-Qin YANG, Rui TUO, Jing WAN, Ming-Xian CHANG, Pin NIE. Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus). Zoological Research, 2014, 35(5): 380-388. doi: 10.13918/j.issn.2095-8137.2014.5.380
Citation: Qiao-Qing XU, Dai-Qin YANG, Rui TUO, Jing WAN, Ming-Xian CHANG, Pin NIE. Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus). Zoological Research, 2014, 35(5): 380-388. doi: 10.13918/j.issn.2095-8137.2014.5.380

Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus)

doi: 10.13918/j.issn.2095-8137.2014.5.380
Funds:  This study was financially supported by the Project from the National Natural Science Foundation of China (31101928), the State Key Laboratory of Freshwater Ecology and Biotechnology (2010FB02) and Public Welfare Scientific Research Project of Hubei Province (2012DBA29001)
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  • Corresponding author: Qiao-Qing XU
  • Received Date: 2013-10-23
  • Rev Recd Date: 2014-04-25
  • Publish Date: 2014-09-08
  • The Asian swamp eel (Monopterus albus) is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel's immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors (IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied. In this study, we characterized IRF4 and IRF10 from M. albus (maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid (aa) protein, while maIRF10 consists of 1 744 nucleotides including an open reading frame (ORF) of 1 236 nt encoding 411 aa. The maIRF10 gene was constitutively expressed at high levels in a variety of tissues, while maIRF4 showed a very limited expression pattern. Expression of maIRF4 and maIRF10 in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.
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Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus)

doi: 10.13918/j.issn.2095-8137.2014.5.380
Funds:  This study was financially supported by the Project from the National Natural Science Foundation of China (31101928), the State Key Laboratory of Freshwater Ecology and Biotechnology (2010FB02) and Public Welfare Scientific Research Project of Hubei Province (2012DBA29001)
    Corresponding author: Qiao-Qing XU

Abstract: The Asian swamp eel (Monopterus albus) is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel's immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors (IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied. In this study, we characterized IRF4 and IRF10 from M. albus (maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid (aa) protein, while maIRF10 consists of 1 744 nucleotides including an open reading frame (ORF) of 1 236 nt encoding 411 aa. The maIRF10 gene was constitutively expressed at high levels in a variety of tissues, while maIRF4 showed a very limited expression pattern. Expression of maIRF4 and maIRF10 in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) and a common pathogenic bacteria Aeromonas hydrophila. These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.

Qiao-Qing XU, Dai-Qin YANG, Rui TUO, Jing WAN, Ming-Xian CHANG, Pin NIE. Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus). Zoological Research, 2014, 35(5): 380-388. doi: 10.13918/j.issn.2095-8137.2014.5.380
Citation: Qiao-Qing XU, Dai-Qin YANG, Rui TUO, Jing WAN, Ming-Xian CHANG, Pin NIE. Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus). Zoological Research, 2014, 35(5): 380-388. doi: 10.13918/j.issn.2095-8137.2014.5.380
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