Molecular identification of <em>Taenia mustelae</em> cysts in subterranean rodent plateau zokors (<em>Eospalax baileyi</em>)

Fang ZHAO; Jun-Ying MA; Hui-Xia CAI; Jian-Ping SU; Zhi-Bin HOU; Tong-Zuo ZHANG; Gong-Hua LIN

doi:10.13918/j.issn.2095-8137.2014.4.313

Volume 35 Issue 4

Jul. 2014

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Fang ZHAO, Jun-Ying MA, Hui-Xia CAI, Jian-Ping SU, Zhi-Bin HOU, Tong-Zuo ZHANG, Gong-Hua LIN. Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi). Zoological Research, 2014, 35(4): 313-318. doi: 10.13918/j.issn.2095-8137.2014.4.313

Citation:

Fang ZHAO, Jun-Ying MA, Hui-Xia CAI, Jian-Ping SU, Zhi-Bin HOU, Tong-Zuo ZHANG, Gong-Hua LIN. Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi). Zoological Research, 2014, 35(4): 313-318. doi: 10.13918/j.issn.2095-8137.2014.4.313

Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

doi: 10.13918/j.issn.2095-8137.2014.4.313

1.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
2.
Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China;
3.
Xining No. 2 People's Hospital of Qinghai Province, Xining 810003, China

Received Date: 2013-07-01
Rev Recd Date: 2014-02-27
Publish Date: 2014-07-08

Abstract

Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors (Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene (cox1) and two nuclear genes (pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.
- Endoparasites,
- New host record,
- Phylogenetic relationships,
- Subterranean rodent

References

[1]	Begall S, Burda H, Schleich CE. 2007. Subterranean Rodents: News from Underground. Berlin: Springer.
[2]	Boev SN, Bondareva VI, Tazieva ZKh. 1971. Vospriimchivost' nekotorykh vidov gryzunov K al' veokokky. Voprosy Prirodnoi Ochagovosti Boleznei, (4): 152-159.
[3]	Cui QH, Lian XM, Zhang TZ, Su JP. 2003. Food habits comparison between Buteo hemilasius and Bubo bubo.Chinese Journal of Zoology, 38(6): 57-63. (in Chinese)
[4]	Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS. 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern. Paris: World Organization for Animal Health and World Health Organization.
[5]	Freeman RS. 1956. Life history studies on Taenia mustelae Gmelin, 1790 and the taxonomy of certain taenioid cestodes from Mustelidae. Canadian Journal of Zoology, 34(4): 219-242.
[6]	Galimberti A, Romano DF, Genchi M, Paoloni D, Vercillo F, Bizzarri L, Sassera D, Bandi C, Genchi C, Ragni B, Casiraghi M. 2012. Integrative taxonomy at work: DNA barcoding of taeniids harboured by wild and domestic cats. Molecular Ecology Resources, 12(3): 403-413.
[7]	Hoberg EP. 2002. Taenia tapeworms: their biology, evolution and socioeconomic significance. Microbes and Infection, 4(8): 859-866.
[8]	Hong L, Lin Y. 1987. Investigation of growth and pathological of E. multilocularis in the human and animals. Endemic Diseases Bulletin, 2(2): 51-61. (in Chinese)
[9]	Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A, Ito A. 2011. Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): An inference from nuclear protein-coding genes. Molecular Phylogenetics and Evolution, 61(3): 628-638.
[10]	Lacey EA, Patton J, Cameron G. 2000. Life Underground: the Biology of Subterranean Rodents. Chicago: University of Chicago Press.
[11]	Li W, Zhang G, Huang G, Wang J, Li Z, Li M. 1985. Investigation on infection of the genera Echinococcus in Ningxia. Endemic Diseases Bulletin, 1(2): 131-133. (in Chinese)
[12]	Nakao M, Sako Y, Yokoyama N, Fukunaga M, Ito A. 2000. Mitochondrial genetic code in cestodes. Molecular and Biochemical Parasitology, 111(2): 415-424.
[13]	Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, Ito A. 2010. State-of-the-art Echinococcus and Taenia: Phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infection Genetics and Evolution, 10(4): 444-452.
[14]	Nevo E. 1999. Mosaic Evolution of Subterranean Mammals: Regression, Progression, and Global Convergence. New York: Oxford University Press.
[15]	Posada D, Crandall KA. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14(9): 817-818.
[16]	Posada D, Crandall KA. 2001. Selecting the best-fit model of nucleotide substitution. Systematic Biology, 50(4): 580-601.
[17]	Ronquist F, Teslenko M, van Mark PD, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. 2012. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61(3): 539-542.
[18]	Swofford DL. 2002. PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods) 4.0 Beta. Massachusetts: Sinauer Associates.
[19]	Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739.
[20]	Xie JX, Lin GH, Liu CX, Yang CH, Deng XG, Cui XF, Li B, Zhang TZ, Su JP. 2014. Diet selection in overwinter caches of plateau zokor (Eospalax baileyi). Acta Theriologica, 59(2):337-345.
[21]	Yang SM, Wei WH, Yin BF, Fan NC, Zhou WY. 2007. Predation risk and survival game of plateau pika and plateau zokor in alpine meadow ecosystem. Acta Ecologica Sinica, 27(12): 4972-4978. (in Chinese)
[22]	Zhang YM. 1999. Effect of plateau zokor on characters and succession of plant communities in alpine meadow. Zoological Research, 20(6): 435-440. (in Chinese)
[23]	Zheng SW, Zeng JX, Cui RX. 1983. On ecology and energy dynamics of masked polecat (Mustela eversmanni) in Haibei Qinghai Province. Acta Theriologica Sinica, 3(1): 35-46. (in Chinese)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

1. Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;

2. Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China;

3. Xining No. 2 People's Hospital of Qinghai Province, Xining 810003, China

Received Date: 2013-07-01

Rev Recd Date: 2014-02-27

Publish Date: 2014-07-08

Key words:

Abstract: Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors (Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene (cox1) and two nuclear genes (pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.

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Reference (23)

[1]	Begall S, Burda H, Schleich CE. 2007. Subterranean Rodents: News from Underground. Berlin: Springer.
[2]	Boev SN, Bondareva VI, Tazieva ZKh. 1971. Vospriimchivost' nekotorykh vidov gryzunov K al' veokokky. Voprosy Prirodnoi Ochagovosti Boleznei, (4): 152-159.
[3]	Cui QH, Lian XM, Zhang TZ, Su JP. 2003. Food habits comparison between Buteo hemilasius and Bubo bubo.Chinese Journal of Zoology, 38(6): 57-63. (in Chinese)
[4]	Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS. 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals: a Public Health Problem of Global Concern. Paris: World Organization for Animal Health and World Health Organization.
[5]	Freeman RS. 1956. Life history studies on Taenia mustelae Gmelin, 1790 and the taxonomy of certain taenioid cestodes from Mustelidae. Canadian Journal of Zoology, 34(4): 219-242.
[6]	Galimberti A, Romano DF, Genchi M, Paoloni D, Vercillo F, Bizzarri L, Sassera D, Bandi C, Genchi C, Ragni B, Casiraghi M. 2012. Integrative taxonomy at work: DNA barcoding of taeniids harboured by wild and domestic cats. Molecular Ecology Resources, 12(3): 403-413.
[7]	Hoberg EP. 2002. Taenia tapeworms: their biology, evolution and socioeconomic significance. Microbes and Infection, 4(8): 859-866.
[8]	Hong L, Lin Y. 1987. Investigation of growth and pathological of E. multilocularis in the human and animals. Endemic Diseases Bulletin, 2(2): 51-61. (in Chinese)
[9]	Knapp J, Nakao M, Yanagida T, Okamoto M, Saarma U, Lavikainen A, Ito A. 2011. Phylogenetic relationships within Echinococcus and Taenia tapeworms (Cestoda: Taeniidae): An inference from nuclear protein-coding genes. Molecular Phylogenetics and Evolution, 61(3): 628-638.
[10]	Lacey EA, Patton J, Cameron G. 2000. Life Underground: the Biology of Subterranean Rodents. Chicago: University of Chicago Press.
[11]	Li W, Zhang G, Huang G, Wang J, Li Z, Li M. 1985. Investigation on infection of the genera Echinococcus in Ningxia. Endemic Diseases Bulletin, 1(2): 131-133. (in Chinese)
[12]	Nakao M, Sako Y, Yokoyama N, Fukunaga M, Ito A. 2000. Mitochondrial genetic code in cestodes. Molecular and Biochemical Parasitology, 111(2): 415-424.
[13]	Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, Ito A. 2010. State-of-the-art Echinococcus and Taenia: Phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infection Genetics and Evolution, 10(4): 444-452.
[14]	Nevo E. 1999. Mosaic Evolution of Subterranean Mammals: Regression, Progression, and Global Convergence. New York: Oxford University Press.
[15]	Posada D, Crandall KA. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14(9): 817-818.
[16]	Posada D, Crandall KA. 2001. Selecting the best-fit model of nucleotide substitution. Systematic Biology, 50(4): 580-601.
[17]	Ronquist F, Teslenko M, van Mark PD, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. 2012. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61(3): 539-542.
[18]	Swofford DL. 2002. PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods) 4.0 Beta. Massachusetts: Sinauer Associates.
[19]	Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739.
[20]	Xie JX, Lin GH, Liu CX, Yang CH, Deng XG, Cui XF, Li B, Zhang TZ, Su JP. 2014. Diet selection in overwinter caches of plateau zokor (Eospalax baileyi). Acta Theriologica, 59(2):337-345.
[21]	Yang SM, Wei WH, Yin BF, Fan NC, Zhou WY. 2007. Predation risk and survival game of plateau pika and plateau zokor in alpine meadow ecosystem. Acta Ecologica Sinica, 27(12): 4972-4978. (in Chinese)
[22]	Zhang YM. 1999. Effect of plateau zokor on characters and succession of plant communities in alpine meadow. Zoological Research, 20(6): 435-440. (in Chinese)
[23]	Zheng SW, Zeng JX, Cui RX. 1983. On ecology and energy dynamics of masked polecat (Mustela eversmanni) in Haibei Qinghai Province. Acta Theriologica Sinica, 3(1): 35-46. (in Chinese)

Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

doi: 10.13918/j.issn.2095-8137.2014.4.313

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Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

doi: 10.13918/j.issn.2095-8137.2014.4.313

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Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

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