Molecular identification of <em>Taenia mustelae</em> cysts in subterranean rodent plateau zokors (<em>Eospalax baileyi</em>)

Fang ZHAO; Jun-Ying MA; Hui-Xia CAI; Jian-Ping SU; Zhi-Bin HOU; Tong-Zuo ZHANG; Gong-Hua LIN

doi:10.13918/j.issn.2095-8137.2014.4.313

Volume 35 Issue 4

Jul. 2014

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Fang ZHAO, Jun-Ying MA, Hui-Xia CAI, Jian-Ping SU, Zhi-Bin HOU, Tong-Zuo ZHANG, Gong-Hua LIN. Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi). Zoological Research, 2014, 35(4): 313-318. doi: 10.13918/j.issn.2095-8137.2014.4.313

Citation:

Fang ZHAO, Jun-Ying MA, Hui-Xia CAI, Jian-Ping SU, Zhi-Bin HOU, Tong-Zuo ZHANG, Gong-Hua LIN. Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi). Zoological Research, 2014, 35(4): 313-318. doi: 10.13918/j.issn.2095-8137.2014.4.313

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Molecular identification of Taenia mustelae cysts in subterranean rodent plateau zokors (Eospalax baileyi)

doi: 10.13918/j.issn.2095-8137.2014.4.313

1.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
2.
Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China;
3.
Xining No. 2 People's Hospital of Qinghai Province, Xining 810003, China

Received Date: 2013-07-01
Rev Recd Date: 2014-02-27
Publish Date: 2014-07-08

Abstract

Abstract

Cestode larvae spend one phase of their two-phase life cycle in the viscera of rodents, but cases of cestodes infecting subterranean rodents have only been rarely observed. To experimentally gain some insight into this phenomenon, we captured approximately 300 plateau zokors (Eospalax baileyi), a typical subterranean rodent inhabiting the Qinghai-Tibet Plateau, and examined their livers for the presence of cysts. Totally, we collected five cysts, and using a mitochondrial gene (cox1) and two nuclear genes (pepck and pold) as genetic markers, we were able to analyze the taxonomy of the cysts. Both the maximum likelihood and Bayesian methods showed that the cysts share a monophyly with Taenia mustelae, while Kimura 2-parameter distances and number of different sites between our sequences and T. mustelae were far less than those found between the examined sequences and other Taeniidae species. These results, alongside supporting paraffin section histology, imply that the cysts found in plateau zokors can be regarded as larvae of T. mustelae, illustrating that zokors are a newly discovered intermediate host record of this parasite.
- Endoparasites,
- New host record,
- Phylogenetic relationships,
- Subterranean rodent

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References(23)

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