cDNA clone and expression analysis of &alpha;-Tropomyosin during Japanese flounder (<em>Paralichthys olivaceus</em>) metamorphosis

Hong-Mei ZHANG; Yan-Fang SU; Zhi-Yi SHI; Yuan-Shuai FU

doi:10.13918/j.issn.2095-8137.2014.4.307

Volume 35 Issue 4

Jul. 2014

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Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307

Citation:

Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307

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cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis

doi: 10.13918/j.issn.2095-8137.2014.4.307

Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China

Received Date: 2014-04-01
Rev Recd Date: 2014-04-01
Publish Date: 2014-07-08

Abstract

Abstract

Tropomyosin (TM) plays a critical role in skeletal and cardiac muscle development and function. To assess the functional significance of α-TM in Japanese flounder (Paralichthys olivaceus) development and metamorphosis, cDNA from Japanese flounder was cloned and α-TM mRNA measured during development and metamorphosis. The full-length cDNA is 1 191 bp, including a 5'-untranslated region of 114 bp, a 3'-UTR of 222 bp, and an open reading frame of 855 bp encoding a polypeptide of 284 amino acids. Real-time quantitative PCR revealed that α-TM mRNA is initially expressed in unfertilized ovum, indicating the α-TM gene is maternal. Relatively low mRNA levels were observed in different embryonic stages. A higher level of α-TM mRNA was detected 3 days post hatching (dph), while the highest level was measured at 29 dph (metamorphic climax) after which it declined towards the end of metamorphosis. The expression of α-TM mRNA was up-regulated in thyroid hormone-treated larvae at 36 dph, but there was no marked difference at other stages when compared to control animals. After thiourea treatment, the expression of α-TM mRNA declined slightly. These data provide basic information that can be utilized in further studies into the role of α-TM in P. olivaceus development and metamorphosis.
- Paralichthys olivaceus,
- Cloning,
- Expression,
- &alpha,
- -Tropomyosin,
- Thyroid hormone

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References(20)

References

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