Advanced Search
Volume 35 Issue 4
Jul.  2014
Turn off MathJax
Article Contents
Article Navigation >  Zoological Research  > 2014  >  35(4): 307-312

Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307
Citation: Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307
shu

cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis

doi: 10.13918/j.issn.2095-8137.2014.4.307

  • Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China

  • Received Date: 2014-04-01
  • Rev Recd Date: 2014-04-01
  • Publish Date: 2014-07-08
  • Tropomyosin (TM) plays a critical role in skeletal and cardiac muscle development and function. To assess the functional significance of α-TM in Japanese flounder (Paralichthys olivaceus) development and metamorphosis, cDNA from Japanese flounder was cloned and α-TM mRNA measured during development and metamorphosis. The full-length cDNA is 1 191 bp, including a 5'-untranslated region of 114 bp, a 3'-UTR of 222 bp, and an open reading frame of 855 bp encoding a polypeptide of 284 amino acids. Real-time quantitative PCR revealed that α-TM mRNA is initially expressed in unfertilized ovum, indicating the α-TM gene is maternal. Relatively low mRNA levels were observed in different embryonic stages. A higher level of α-TM mRNA was detected 3 days post hatching (dph), while the highest level was measured at 29 dph (metamorphic climax) after which it declined towards the end of metamorphosis. The expression of α-TM mRNA was up-regulated in thyroid hormone-treated larvae at 36 dph, but there was no marked difference at other stages when compared to control animals. After thiourea treatment, the expression of α-TM mRNA declined slightly. These data provide basic information that can be utilized in further studies into the role of α-TM in P. olivaceus development and metamorphosis.
  • 加载中
  • [1] Bing W, Knott A, Redwood C, Esposito G, Purcell I, Watkins H, Marston S. 2000. Effect of hypertrophic cardiomyopathy mutations in human cardiac muscle α-tropomyosin (Asp175Asn and Glu180Gly) on the regulatory properties of human cardiac troponin determined by in vitro motility assay. Journal of Molecular and Cellular Cardiology, 32(8): 1489-1498.
    [2] Fu YS, Shi ZY, Wang GY, Li WJ, Zhang JL, Jia L. 2012. Expression and regulation of miR-1,-133a,-206a, and MRFs by thyroid hormone during larval development in Paralichthys olivaceus. Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology, 161(3): 226-232.
    [3] Gordon AM, Homsher E, Regnier M. 2000. Regulation of contraction in striated muscle. Physiological Reviews, 80(2): 853-924.
    [4] Inui Y, Miwa S. 1985. Thyroid hormone induces metamorphosis of flounder larvae. General and Comparative Endocrinology, 60(3): 450-454.
    [5] Jagatheesan G, Rajan S, Wieczorek DF. 2010. Investigations into tropomyosin function using mouse models. Journal of Molecular and Cellular Cardiology, 48(5): 893-898.
    [6] Kirwan JP, Hodges RS. 2010. Critical interactions in the stability control region of tropomyosin. Journal of Structural Biology, 170(2): 294-306.
    [7] Li XY, Lin YS, Zhang HW. 2012. Phylogenetic analysis and expression patterns of tropomyosin in amphioxus. Zoological Research, 33(4): 389-394. (in Chinese)
    [8] Marston S, Memo M, Messer A, Papadaki M, Nowak K, McNamara E, Ong R, El-Mezgueldi M, Li XC, Lehman W. 2013. Mutations in repeating structural motifs of tropomyosin cause gain of function in skeletal muscle myopathy patients. Human Molecular Genetics, 22(24): 4978-4987.
    [9] Minami T. 1982. The early life history of a flounder Paralichthys olivaceus [in Wakasa Bay, Japan Sea, Japan]. Bulletin of the Japanese Society of Scientific Fisheries, 48(11):1581-1588.
    [10] Muthuchamy M, Pajsk L, Howles P, Doetschman T, Wieczorek DF.
    [11] 1993. Developmental analysis of tropomyosin gene expression in embryonic stem cells and mouse embryos. Molecular and Cellular Biology, 13(6): 3311-3323.
    [12] Pearlstone JR, Smillie LB. 1983. Effects of troponin-I plus-C on the binding of troponin-T and its fragments to alpha-tropomyosin. Ca2+ sensitivity and cooperativity. Journal of Biological Chemistry, 258(4): 2534-2542.
    [13] Rethinasamy P, Muthuchamy M, Hewett T, Boivin G, Wolska BM, Evans C, Solaro RJ, Wieczorek DF. 1998. Molecular and physiological effects of α-tropomyosin ablation in the mouse. Circulation Research, 82(1): 116-123.
    [14] Schefe JH, Lehmann KE, Buschmann IR, Unger T, Funke-Kaiser H. 2006. Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression's CT difference” formula. Journal of Molecular Medicine, 84(11): 901-910.
    [15] Schreiber AM & Specker JL. 1998. Metamorphosis in the Summer Flounder (Paralichthys dentatus): Stage-Specific Developmental Response to Altered Thyroid Status. General and comparative endocrinology, 111(2): 156-166.
    [16] Sumida JP, Wu E, Lehrer SS. 2008. Conserved Asp-137 imparts flexibility to tropomyosin and affects function. Journal of Biological Chemistry, 283(11): 6728-6734.
    [17] Wilbur HM. 1980. Complex life cycles. Annual review of Ecology and Systematics, 11: 67-93.
    [18] Yamano K, Miwa S, Obinata T, Inui Y. 1991. Thyroid hormone regulates developmental changes in muscle during flounder metamorphosis. General and Comparative Endocrinology, 81(3): 464-472.
    [19] Yamano K, Takano-Ohmuro H, Obinata T, Inui Y. 1994. Effect of thyroid hormone on developmental transition of myosin light chains during flounder metamorphosis. General and Comparative Endocrinology, 93(3): 321-326.
    [20] Youson JH. 1988. First metamorphosis. Fish Physiology, 11(Part B): 135-196.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(493) PDF downloads(1284) Cited by()

Related
Proportional views

cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis

doi: 10.13918/j.issn.2095-8137.2014.4.307
  • Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China
  • Received Date: 2014-04-01
  • Rev Recd Date: 2014-04-01
  • Publish Date: 2014-07-08

Abstract: Tropomyosin (TM) plays a critical role in skeletal and cardiac muscle development and function. To assess the functional significance of α-TM in Japanese flounder (Paralichthys olivaceus) development and metamorphosis, cDNA from Japanese flounder was cloned and α-TM mRNA measured during development and metamorphosis. The full-length cDNA is 1 191 bp, including a 5'-untranslated region of 114 bp, a 3'-UTR of 222 bp, and an open reading frame of 855 bp encoding a polypeptide of 284 amino acids. Real-time quantitative PCR revealed that α-TM mRNA is initially expressed in unfertilized ovum, indicating the α-TM gene is maternal. Relatively low mRNA levels were observed in different embryonic stages. A higher level of α-TM mRNA was detected 3 days post hatching (dph), while the highest level was measured at 29 dph (metamorphic climax) after which it declined towards the end of metamorphosis. The expression of α-TM mRNA was up-regulated in thyroid hormone-treated larvae at 36 dph, but there was no marked difference at other stages when compared to control animals. After thiourea treatment, the expression of α-TM mRNA declined slightly. These data provide basic information that can be utilized in further studies into the role of α-TM in P. olivaceus development and metamorphosis.

Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307
Citation: Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312. doi: 10.13918/j.issn.2095-8137.2014.4.307
shu

    HTML

Reference (20)

Catalog

    Content

    For Authors

    About ZR

    Copyright © 2016 Editorial Office of ZOOLOGICAL RESEARCH

    滇ICP备05000723号-2滇公网安备 53010202000944号

    Supported by: Beijing Renhe Information Technology Co. Ltd  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return