Volume 35 Issue 4
Jul.  2014
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Yu-Mei TIAN, Jie CHEN, Yang TAO, Xia-Yun JIANG, Shu-Ming ZOU. Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(4): 300-306. doi: 10.13918/j.issn.2095-8137.2014.4.300
Citation: Yu-Mei TIAN, Jie CHEN, Yang TAO, Xia-Yun JIANG, Shu-Ming ZOU. Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(4): 300-306. doi: 10.13918/j.issn.2095-8137.2014.4.300

Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala)

doi: 10.13918/j.issn.2095-8137.2014.4.300
  • Received Date: 2013-09-29
  • Rev Recd Date: 2013-12-26
  • Publish Date: 2014-07-08
  • Insulin-like growth factor-binding protein 1 (IGFBP-1), a hypoxia-induced protein, is a member of the IGFBP family that regulates vertebrate growth and development. In this study, full-length IGFBP-1a cDNA was cloned from a hypoxia-sensitive Cyprinidae fish species, the blunt snout bream (Megalobrama amblycephala). IGFBP-1a was expressed in various organs of adult blunt snout bream, including strongly in the liver and weakly in the gonads. Under hypoxia, IGFBP-1a mRNA levels increased sharply in the skin, liver, kidney, spleen, intestine and heart tissues of juvenile blunt snout bream, but recovered to normal levels after 24-hour exposure to normal dissolved oxygen. In blunt snout bream embryos, IGFBP-1a mRNA was expressed at very low levels at both four and eight hours post-fertilization, and strongly at later stages. Embryonic growth and development rates decreased significantly in embryos injected with IGFBP-1a mRNA. The average body length of IGFBP-1a-overexpressed embryos was 82.4% of that of the control group, and somite numbers decreased to 85.2%. These findings suggest that hypoxia-induced IGFBP-1a may inhibit growth in this species under hypoxic conditions.
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Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala)

doi: 10.13918/j.issn.2095-8137.2014.4.300

Abstract: Insulin-like growth factor-binding protein 1 (IGFBP-1), a hypoxia-induced protein, is a member of the IGFBP family that regulates vertebrate growth and development. In this study, full-length IGFBP-1a cDNA was cloned from a hypoxia-sensitive Cyprinidae fish species, the blunt snout bream (Megalobrama amblycephala). IGFBP-1a was expressed in various organs of adult blunt snout bream, including strongly in the liver and weakly in the gonads. Under hypoxia, IGFBP-1a mRNA levels increased sharply in the skin, liver, kidney, spleen, intestine and heart tissues of juvenile blunt snout bream, but recovered to normal levels after 24-hour exposure to normal dissolved oxygen. In blunt snout bream embryos, IGFBP-1a mRNA was expressed at very low levels at both four and eight hours post-fertilization, and strongly at later stages. Embryonic growth and development rates decreased significantly in embryos injected with IGFBP-1a mRNA. The average body length of IGFBP-1a-overexpressed embryos was 82.4% of that of the control group, and somite numbers decreased to 85.2%. These findings suggest that hypoxia-induced IGFBP-1a may inhibit growth in this species under hypoxic conditions.

Yu-Mei TIAN, Jie CHEN, Yang TAO, Xia-Yun JIANG, Shu-Ming ZOU. Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(4): 300-306. doi: 10.13918/j.issn.2095-8137.2014.4.300
Citation: Yu-Mei TIAN, Jie CHEN, Yang TAO, Xia-Yun JIANG, Shu-Ming ZOU. Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(4): 300-306. doi: 10.13918/j.issn.2095-8137.2014.4.300
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