1988 Vol. 9, No. 1
Recurrence of sexual gland in relation to winter flocking in sparrows was studied by prolonging the daily photoperiod during sexual resting period in experimental populations of sparrows. Results showed that prolonged daily photoperiod did have an effect on recurrence of sexual gland, the degree of recurrence, however, not noly depended on the duration of prolonged daily photoperiod, but also on the status of flocking. Under certain conditions of photoperiod, the recurrence of sexual gland in flocking birds was more significant than that in the isolated single birds, which suggested that winter flocking in sparrow would be an essential process for later reproduction.
Detergents, Body cells, Germ cells, Micronuclei, Chromosomal aberrations, SCEs
It's the first time that the study on mitotic karyotype of ghost moth was reported in this paper. Using an acetic acid dissociation and air-drying technique, we have studied the mitotic chromosomes of two species of ghost moth in Ynnan Province of China, Hepialus zhayuensis Chu et Wang and Hepialus sp. (Lepidoptera, Hepialidae).Their diploid chromosome number is 2n=64. The holocemtric is clearly shown on chromosomes at early mitotic metaphase. However, in mitotic metaphase and later metaphase, the primary centromeres (primary consirictions) can be seen on each chromosome. A pair of typical heteromorphic sex chromosomes were found in the male karyotype of two species. X-chromosome is provided with metacentric or submetacentric and more slightly seained. Y-chromosome is deeply stained and bigger than the other chromosomes in the karyotype.In male interphase cell, one heteropycnotic sex chromatin body can be found distinctly. That is the Y-chromosome.
This articel dealt with the studies on chromosome aberration induced with 60Co gamma ray in a species of gamasid. Eulaelaps shanghaiensis (Acari, Gamasina). Ghromosome aberration of the gamasid mite egg cells following differnet doses of 60Co gamma ray(1-50 Krad) had been studied. Chromosome gap(csg), fragments(f), minute (min), ring chromosome (r), pulverization (prz) and polyploid were found. Chromosome fragments are most frequent chromosome aberration. micronuclei were also formed. The rates of chromosome aberrations were increased with the radiation dose. By means of an analysis of statistics, a curvilinear regression relationship between irradiation dose and chromosome aberration rate, Y=3.27+14.49lg(X+1), was revealed.
The concentration of sex steroids in the serum of 7 females rhesus monkeys during the intermenstrual period were measured by radioimmunoassay. Fifteen of 20 menstrual cycles have s surge of estradiol and testosterone in the middle cycles. The average surge value of estradiol and testosterone are 179.1±91.3pg/ml and 1010.1±411.3pg/ml respectively. In normal luteal phase, the value of progesterone is not below 1ng/ml. The average length of 20 menstrual cycles is 28.6±5.4 days (mean±SD). Follicle phase is 11.9±2.8 days. Luteal phase is 19.2±6.3days. The menstrual cycles are expressed by the formua Y=18.92±0.03X²(Y: menstrual cycle, X: luteal phase; R=0.9444). The results suggest that musuring the three steroids hormones can be accurately indexed ovulatory in rhesus monkeys. The mechanism of secretion and regulation of testosterone in reproductive cycle will be studied continuously.
1988, 9(1): 45-60.
The tryptophan, arginine and lysine residues of cecropin D were modified by N-bromosuccinimide (NBS), 1, 2-CYCLOHEXANEDIONE (CHD) and maleic little relationship to the antibacterial activity of the peptide against Escherichiacoli D31, but that the arginine and lysine residues were important to the cecropin D resulted in a total loss of its antibacterial activity against E. coli D31. When the arginine and lysine residues were each regenerated from the modified structures the antibacterial acivity of cecropin D was recovered. These results suggest that the antibacterial activity of cecropin D is related to the charge in the molecule. Ouchterlony double immunodiffusion showed that the antigenic determinant of cecropin D is closely connected with the arginine residues in the peptide.
The isozyme patterns and relative mobile characteristic of lactate dehydrogenase (LDH) and esterase from various organs and tissues of Carassius auratus gibelio Bloch, Carassius auratus, Carassius sp., have been comparatively studied by vertical polyacrylamide electrophoresis method.Specific isozyme patterns were observed in the serum, lence , brain, heart, kidney, spleen, liver, muscle, ovary of Carassius sp. The results showed significant tissue-specific difference in esterase zymograms. There are 3 types of phenotypes.Our results indicated that zymogram for LDH isozyme activity in the some organs of carassius auratus gibelio Bloch and Carassius sp. showed significant differences in hand numbers and their strngth and relative mobilities. The results of disc polyacrylamide gel isoelectric focusing(T7.5%, C 5%) showed that zymogrm and electrophoresis densitometric scans for Est. isozyme in the same organs between the two kinds of fishes displayed clear differences (liver, ovary, brain).These results showed some differentiation already at biological level in Carassius auratus gibelio Bloch and Carassius sp. They possibly originated from different regions and developed independently form different ancestors. LDH zymogram of Carassius sp. and Carassius auratus were similar. It seems that Carassius sp. originated possibly from native Carassius auratus of Yunnan Province.
In this experiment, 3µg of colchicine were respectively injected into varous parts of the bilateral hippocampal fromation in adult Wistar's female rats. We observed whether it could influence the learning and memory of dark avoidance behavior, and examined correlative histology. These results show that the rats having been injured the granual cells of bilateral dorsal or posterior dorsal parts of dentate gyrus can perform the learning and memory of the dark avoidance behavior without any impairment, but injured at ventral part from H6.0mm horizontal plane in rat's brain stereotaxic atlas by Krieg, they perform it with strikingly weakened, however don't block it entirely.
The present paper deals with the morphological structure of the swim bladder and air-breathing in Pangasius sutchi (Fowler), a freshwater catfish introduced from Thailand into China in 1978. the swim bladder of P. sutchi is a well vascularized vesicular organ with closely concentre respiratory cells like lung-aviolus. In normal conditon, the fish goes to the water surface and exchanges gases with the atmosphere to get oxygen at inervals.The air-breathing frequency is determined to be 17.2-17.5 per fish. hr. when the dissolved oxygen content is 0.75-0.65mg/l, temperature is 25.2-25.8℃, the frequency decreases as the level of dissolved oxgen content in water increases. At high level of dissolved oxygen content in water (3.45-5.46mg/l), air-breathing still exists, but less frequently. When surfacing activity is prevented, the suffocation point is 0.95-1.06mg/l, which much higher than swim bladder is confirmed to be an important accessory respiratory organ which makes the fish great adaptability to the circumstances. Such a special rogan is scarely found in catfish.
Our experiments have shown that the white wax insect bred in Yunnan, Sichuan and Guizhou province secretes much more wax than that bred in other areas. So we propse that department responsible for white wax production should advocate the development of the production of the insects in the three provinces mentioned above.
lesser panda, spinal cord, grey matter of the cervical segments
Hemorrhagin I (AsHI) isolated from A. acutus venom causes repture of vascular system and extensive hemorrhage. The specific attachment of HRP-AaHI to the endothelial cell by the method of horseradish peroxidase conjugation is reported in this paper. The results suggest that receptor of binding site of hemorrhagin I might be present on the outer-membrane of endothelial cell.