1980 Vol. 1, No. 1

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研究论文
The present paper deals with the fishes referring to the subfamily Botiinae found in the Chinese fresh-water.At present 3 genera,22 species and 2 subspecies of this subfamily have so far been known in China.Among them,Botia (Hymenophysa) yunnanensis,Parabotia lijiangensis,Parabotia bimaculata,Parabotia parua,Leptobotia guilinensis are found to be new to science.
The present paper deals with the larvae of 10 species of Luciliini from China.
This paper presents the comparative studies of the karyotypes of five cattle species.The chromosome number of Bos taurus,B.grunniens and B.indicus are all 2n=60,while those of B.frontalis and B.gaurus readei are 2n=58 and 2n=56 respectively.The diploid numbers and chromosomal morphology of the five species have been compared each other.B.taurus and B.grunniens seem to have the same karyotype.The karyotype of B.indicus differs both from that of B.taurus and that of B.grunniens only in the presence of the subacrocentric Y.Bos frontalis has a diploid number of 2n=58 with one pair of metacentric autosome.B.gaurus readei possesses the lowest diploid number (2n=56) in the five species of cattles examined.Its karyotype is different from other species because of the presence of two pairs of submetacentric autosomes.Finally,the possibility of hybridization among five species of cattles is discussed from the point of view of cytogenetics.
In the present paper,karyotype of the mouse ascites hepatoma is analysed with several banding techniques.The results show that the mouse ascites hepatoma is near-diploid with a modal number of 42-43 (13.0-32.0%) and four or five marker chromosomes.By Ag-As procedure,silver stained NORs (Ag-NORs) are visualized as black spherical bodies on 2-3 chromosomes in the karyotype of the mouse ascites hepatoma.Ag-NORs in this mouse ascites hepatoma cell are usually seen to occur at secondary constrictions on the M1 and M3 chromosomes.
Karyotypical and banding pattern studies in the four species of monkeys belonging to genus Macaca have been investigated comparatively by a modified microculture method of peripheral blood cells and other related chromosome technique.In all four species,they have not only the same chromosome number of 2n=42,but also identical distribution of G-and C-bands.By measuring of the relative lengths and arm ratio,there also reveals no significant difference in the characteristics of the chromosome complements among the four species.So it shows that the coefficient of relationship among them is very close.
The venom of Agkistrodon halys (From Snake Island) was fractionated on DEAE-Sephadex A-50 into sixteen different fractions.The experimental conditions are described.Some enzymatic activities (Arginine ester hydrolase,proteinase,phosphomonoesterase,phosphodiesterase,L-amino acid oxidase,5-nucleotidase and ribonuclease) as well as lethal,haemorrhagic and bradykinin releasing activities have been determined for each fraction.
The venem of Agkistrodon acutus (From Fukien) was fractionated on DEAE-Sephadex A-50 into 11 different protein peaks (Fractions).The experimental conditions are described.In the crude venom,the specific activities of proteinase,arginine esterase,phosphodiesterase,as well as 5-nucleotidase are higher than that of phosphomonoesterase,phospholipase A and L-amino acid oxidase.The arginineesterase and thrombin-like activities are concentrated in fraction Ⅵ.The proteinase,fibrinolytic and anticoagulant activities are concentrated in fraction Ⅰ.Fraction Ⅸ also shows anticoagulant activity.The lethal action is located in fraction Ⅹ.The LD50 after intraperitoneal injectoin of crude venom of Agkistrodon acutus was found to be 4.4 mg/kg for mice.
P-aminoazobenzene hydrochloride showed a marked radioprotective effect in mice irradiated with lethal doses of [60]Co γ-rays.The effective dose of the drug was 50 mg/kg body weight which was administered either intraperitoneally or per os before irradiation.The advantages of this drug are:Both oral and intraperitoneal routes of administration are effective,the latency of appearance of protective effect is relatively short and the range of effective doses is wide.Furthermore,the chemical properties of this drug is stable under room temperature.On the other hand,the formation of metahemoglobin may be considered as one of the drawbacks of this drug,but within the range of effective doses it is well tolerated by the animals.The mechanism of protection of this drug has been discussed based on our preliminary work elucidating this point.