Volume 36 Issue 3
May  2015
Turn off MathJax
Article Contents

Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.
Citation: Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.

Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection

Funds:  This project was supported by the National 863 Project (2012AA10A403), the Natural Science Foundation of Ningbo City of China (2014A610187) and the Scientific Research Foundation of Graduate School of Ningbo University (G14041)
More Information
  • Corresponding author: Jiong CHEN
  • Received Date: 2015-03-23
  • Rev Recd Date: 2015-04-24
  • Publish Date: 2015-05-08
  • Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass (Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.
  • 加载中
  • [1] Angosto D, Montero J, López-Muñoz A, Alcaraz-Pérez F, Bird S, Sarropoulou E, Abellán E, Meseguer J, Sepulcre MP, Mulero V. 2013. Identification and functional characterization of a new IL-1 family member, IL-1Fm2, in most evolutionarily advanced fish. Innate Immunity, 20(5): 487-500.
    [2] Bird PI, Trapani JA, Villadangos JA. 2009. Endolysosomal proteases and their inhibitors in immunity. Nature Reviews Immunology, 9(12): 871-882.
    [3] Buonocore F, Forlenza M, Randelli E, Benedetti S, Bossù P, Meloni S, Secombes CJ, Mazzini M, Scapigliati G. 2005. Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1b. Marine Biotechnology, 7(6): 609-617.
    [4] Cai ZH, Song LS, Gao CP, Wu LT, Qiu LH. 2004. Molecular cloning and expression of interleukin 1 beta (IL-1β) from red sea bream (Pagrus major). Progress in Natural Science, 14(5): 396-402.
    [5] Carrero R, Cerrada I, Lledó E, Dopazo J, García-García F, Rubio MP, Trigueros C, Dorronsoro A, Ruiz-Sauri A, Montero JA, Sepúlveda P. 2012. IL1β induces mesenchymal stem cells migration and leucocyte chemotaxis through NF-κB. Stem Cell Reviews and Reports, 8(3): 905-916.
    [6] Chen J, Chen Q, Lu XJ, Li CH. 2014. LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages. Fish and Shellfish Immunology, 41(2): 586-592.
    [7] Chen XH, Lin KB, Wang XW. 2003. Outbreaks of an iridovirus disease in maricultured large yellow croaker, Larimichthys crocea (Richardson), in China. Journal of Fish Diseases, 26(10): 615-619.
    [8] Corripio-Miyar Y, Bird S, Tsamopoulos K, Secombes CJ. 2007. Cloning and expression analysis of two pro-inflammatory cytokines, IL-1b and IL-8, in haddock (Melanogrammus aeglefinus). Molecular Immunology, 44(6): 1361-1373.
    [9] Descamps D, Le Gars M, Balloy V, Barbier D, Maschalidi S, Tohme M, Chignard M, Ramphal R, Manoury B, Sallenave JM. 2012. Toll-like receptor 5 (TLR5), IL-1b secretion, and asparagine endopeptidase are critical factors for alveolar macrophage phagocytosis and bacterial killing. Proceedings of the National Academy of Sciences of the United States of America, 109(5): 1619-1624.
    [10] Ebisawa M, Bochner BS, Georas SN, Schleimer RP. 1992. Eosinophil transendothelial migration induced by cytokines. I. Role of endothelial and eosinophil adhesion molecules in IL-1 beta-induced transendothelial migration. The Journal of Immunology, 149(12): 4021-4028.
    [11] Fujiki K, Shin DH, Nakao M, Yano T. 2000. Molecular cloning and expression analysis of carp (Cyprinus carpio) interleukin-1b, high affinity immunoglobulin E Fc receptor γ subunit and serum amyloid A. Fish and Shellfish Immunology, 10(3): 229-242.
    [12] Hong S, Peddie S, Campos-Pérez JJ, Zou J, Secombes CJ. 2003. The effect of intraperitoneally administered recombinant IL-1b on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss). Developmental and Comparative Immunology, 27(9): 801-812.
    [13] Hong S, Zou J, Crampe M, Peddie S, Scapigliati G, Bols N, Cunningham C, Secombes CJ. 2001. The production and bioactivity of rainbow trout (Oncorhynchus mykiss) recombinant IL-1b. Veterinary Immunology and Immunopathology, 81(1-2): 1-14.
    [14] Husain M, Bird S, Van Zwieten R, Secombes CJ, Wang T. 2012. Cloning of the IL-1b3 gene and IL-1b4 pseudogene in salmonids uncovers a second type of IL-1b gene in teleost fish. Developmental and Comparative Immunology, 38(3): 431-446.
    [15] Kono T, Fujiki K, Nakao M, Yano T, Endo M, Sakai M. 2002. The immune responses of common carp, Cyprinus carpio L., injected with carp interleukin-1b gene. Journal of Interferon & Cytokine Research, 22(4): 413-419.
    [16] Li HX, Lu XJ, Li CH, Chen J. 2014. Molecular characterization and functional analysis of two distinct liver-expressed antimicrobial peptide 2 (LEAP-2) genes in large yellow croaker (Larimichthys crocea). Fish and Shellfish Immunology, 38(2): 330-339.
    [17] Li SY, Ao JQ, Chen XH. 2009. Molecular and functional characterization of a cystatin analogue in large yellow croaker (Pseudosciaena crocea). Molecular Immunology, 46(8-9): 1638-1646.
    [18] Lu XJ, Chen J, He YQ, Shi YH. 2013. Molecular characterization of an IL-1b gene from ayu, Plecoglossus altivelis. Fish and Shellfish Immunology, 34(5): 1253-1259.
    [19] Matsukawa A, Hogaboam CM, Lukacs NW, Lincoln PM, Evanoff HL, Kunkel SL. 2000. Pivotal role of the CC chemokine, macrophage-derived chemokine, in the innate immune response. The Journal of Immunology, 164(10): 5362-5368.
    [20] Mu YN, Wan X, Lin KB, Ao JQ, Chen XH. 2013. Liver proteomic analysis of the large yellow croaker (Pseudosciaena crocea) following polyriboinosinic: polyribocytidylic acid induction. Fish Physiology and Biochemistry, 39(5): 1267-1276.
    [21] Peddie S, Zou J, Cunningham C, Secombes CJ. 2001. Rainbow trout (Oncorhynchus mykiss) recombinant IL-1b and derived peptides induce migration of head-kidney leucocytes in vitro. Fish and Shellfish Immunology, 11(8): 697-709.
    [22] Peddie S, Zou J, Secombes CJ. 2002. A biologically active IL-1b derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro. Journal of Fish Diseases, 25(6): 351-360.
    [23] Reis MIR, Do Vale A, Pereira PJ, Azevedo JE, Dos Santos NM. 2012. Caspase-1 and IL-1β processing in a teleost fish. PLoS One, 7(11): e50450.
    [24] Rodenburg RJ, Brinkhuis RF, Peek R, Westphal J, Van Den Hoogen FH, Van Venrooij WJ, Van De Putte LB. 1998. Expression of macrophage-derived chemokine (MDC) mRNA in macrophages is enhanced by interleukin-1b, tumor necrosis factor alpha, and lipopolysaccharide. Journal of Leukocyte Biology, 63(5): 606-611.
    [25] Scapigliati G, Buonocore F, Bird S, Zou J, Pelegrin P, Falasca C, Prugnoli D, Secombes CJ. 2001. Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1b. Fish and Shellfish Immunology, 11(8): 711-726.
    [26] Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739.
    [27] Wang TH, Bird S, Koussounadis A, Holland JW, Carrington A, Zou J, Secombes CJ. 2009. Identification of a novel IL-1 cytokine family member in teleost fish. The Journal of Immunology, 183(2): 962-974.
    [28] Yu Y, Zhong QW, Li CM, Jiang LM, Sun YY, Wang XB, Wang ZG, Zhang QQ. 2012. Molecular cloning and characterization of interleukin-1b in half-smooth tongue sole Cynoglossus semilaevis. Veterinary Immunology and Immunopathology, 146(3-4): 270-276.
    [29] Zhang RC, Chen J, Li CH, Lu XJ, Shi YH. 2011. Prokaryotic expression, purification, and refolding of leukocyte cell-derived chemotaxin 2 and its effect on gene expression of head kidney-derived macrophages of a teleost fish, ayu (Plecoglossus altivelis). Fish and shellfish Immunology, 31(6): 911-918.
    [30] Zou J, Grabowski PS, Cunningham C, Secombes CJ. 1999. Molecular cloning of interleukin 1b from rainbow trout Oncorhynchus mykiss reveals no evidence of an ICE cut site. Cytokine, 11(8): 552-560.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(572) PDF downloads(1175) Cited by()

Related
Proportional views

Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection

Funds:  This project was supported by the National 863 Project (2012AA10A403), the Natural Science Foundation of Ningbo City of China (2014A610187) and the Scientific Research Foundation of Graduate School of Ningbo University (G14041)
    Corresponding author: Jiong CHEN

Abstract: Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass (Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.

Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.
Citation: Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.
Reference (30)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return