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Yongtang Zheng, Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian. Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.049
Citation: Yongtang Zheng, Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian. Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.049

Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research

doi: 10.24272/j.issn.2095-8137.2020.049
  • Received Date: 2020-01-17
  • Accepted Date: 2020-05-06
  • Rev Recd Date: 2020-04-29
  • Available Online: 2020-05-21
  • Intestinal biopsy is a basic experimental method for studying pathological changes in the intestinal tract during human immunodeficiency virus (HIV) infection. In this study, jejunal resection and anastomosis were successfully performed in 12 Chinese rhesus macaques (Macaca mulatta). The sampled gut tissues were then examined by hematoxylin and eosin (H&E) staining, electron microscopy, flow cytometry, immunofluorescence detection, and RNA quality analysis to ensure suitability for histological, physiological, pathological, and immunological detection, as well as mechanistic analysis at the cellular and molecular level. Importantly, the surgery did not affect the ratio or number of immune cells in peripheral blood or the concentration of lipids, proteins, and vitamins in plasma, which are important indicators of nutritional status. Our results thus indicated that jejunal resection and anastomosis are feasible, and that immune homeostasis and intestinal barrier integrity are not altered by surgery. All macaques recovered well (except for one), with no postoperative complications. Therefore, this animal surgery may be applicable for longitudinal intestinal research related to diseases such as acquired immunodeficiency syndrome (AIDS).
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research

doi: 10.24272/j.issn.2095-8137.2020.049

Abstract: Intestinal biopsy is a basic experimental method for studying pathological changes in the intestinal tract during human immunodeficiency virus (HIV) infection. In this study, jejunal resection and anastomosis were successfully performed in 12 Chinese rhesus macaques (Macaca mulatta). The sampled gut tissues were then examined by hematoxylin and eosin (H&E) staining, electron microscopy, flow cytometry, immunofluorescence detection, and RNA quality analysis to ensure suitability for histological, physiological, pathological, and immunological detection, as well as mechanistic analysis at the cellular and molecular level. Importantly, the surgery did not affect the ratio or number of immune cells in peripheral blood or the concentration of lipids, proteins, and vitamins in plasma, which are important indicators of nutritional status. Our results thus indicated that jejunal resection and anastomosis are feasible, and that immune homeostasis and intestinal barrier integrity are not altered by surgery. All macaques recovered well (except for one), with no postoperative complications. Therefore, this animal surgery may be applicable for longitudinal intestinal research related to diseases such as acquired immunodeficiency syndrome (AIDS).

Yongtang Zheng, Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian. Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.049
Citation: Yongtang Zheng, Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian. Successful Implementation of Intestinal Resection and Anastomosis in Non-human Primates suggests the Possibility of Longitudinal Intestinal Research[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.049
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