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Luying Wang, Zixu Liu, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Weiqiang Zheng, Fan Wang, Qingpi Yan. Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.048
Citation: Luying Wang, Zixu Liu, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Weiqiang Zheng, Fan Wang, Qingpi Yan. Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.048

Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)

doi: 10.24272/j.issn.2095-8137.2020.048
  • Received Date: 2020-02-20
  • Accepted Date: 2020-04-21
  • Rev Recd Date: 2020-04-14
  • Available Online: 2020-05-21
  • Pseudomonas plecoglossicida is a Gram-negative bacterium that is rod-shaped and has flagella. This bacterium causes visceral white spot disease and a very high mortality rate during the culture of Larimichthys crocea, which can cause serious economic losses. Analysis of transcriptome data and quantitative real-time PCR showed that the expression of dksA gene was significantly up-regulated after 48 hours of infection with Epinephelus coioides (log2FC = 3.12, p <0.001). RNAi of five shRNAs significantly reduced the expression of the gene dksA in P. plecoglossicida, and the optimal silencing efficiency was 96.23%. Compared with wild-type strains, the symptoms of visceral white spot disease in L. crocea infected with RNAi strains were reduced, the death time was delayed by 48 hours and the mortality rate was reduced by 20%. The silencing of the dksA gene leads to a large amount of down-regulation of the cellular components, flagella and ribosome assembly-related genes in P. plecoglossicida, and the significant up-regulation of the fliC gene may be a way to maintain the virulence of P. plecoglossicida. GO and KEGG results showed that L. crocea infected by RNAi strains were significantly down-regulated in inflammatory factor genes in immune-related pathways. In these immune pathways, the affected genes were associated with multiple immune response processes. The results showed that dksA was a virulence gene of P. plecoglossicida. Compared with wild strains, RNAi strains infection induced a weaker immune response in L. crocea.
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Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)

doi: 10.24272/j.issn.2095-8137.2020.048

Abstract: Pseudomonas plecoglossicida is a Gram-negative bacterium that is rod-shaped and has flagella. This bacterium causes visceral white spot disease and a very high mortality rate during the culture of Larimichthys crocea, which can cause serious economic losses. Analysis of transcriptome data and quantitative real-time PCR showed that the expression of dksA gene was significantly up-regulated after 48 hours of infection with Epinephelus coioides (log2FC = 3.12, p <0.001). RNAi of five shRNAs significantly reduced the expression of the gene dksA in P. plecoglossicida, and the optimal silencing efficiency was 96.23%. Compared with wild-type strains, the symptoms of visceral white spot disease in L. crocea infected with RNAi strains were reduced, the death time was delayed by 48 hours and the mortality rate was reduced by 20%. The silencing of the dksA gene leads to a large amount of down-regulation of the cellular components, flagella and ribosome assembly-related genes in P. plecoglossicida, and the significant up-regulation of the fliC gene may be a way to maintain the virulence of P. plecoglossicida. GO and KEGG results showed that L. crocea infected by RNAi strains were significantly down-regulated in inflammatory factor genes in immune-related pathways. In these immune pathways, the affected genes were associated with multiple immune response processes. The results showed that dksA was a virulence gene of P. plecoglossicida. Compared with wild strains, RNAi strains infection induced a weaker immune response in L. crocea.

Luying Wang, Zixu Liu, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Weiqiang Zheng, Fan Wang, Qingpi Yan. Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.048
Citation: Luying Wang, Zixu Liu, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Weiqiang Zheng, Fan Wang, Qingpi Yan. Dual RNA-seq provides a novel insight into the roles of dksA gene of Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)[J]. Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.048
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