Volume 41 Issue 4
Jul.  2020
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Lu-Ying Wang, Zi-Xu Liu, Ling-Min Zhao, Li-Xing Huang, Ying-Xue Qin, Yong-Quan Su, Wei-Qiang Zheng, Fan Wang, Qing-Pi Yan. Dual RNA-seq provides novel insight into the roles of dksA from Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea). Zoological Research, 2020, 41(4): 410-422. doi: 10.24272/j.issn.2095-8137.2020.048
Citation: Lu-Ying Wang, Zi-Xu Liu, Ling-Min Zhao, Li-Xing Huang, Ying-Xue Qin, Yong-Quan Su, Wei-Qiang Zheng, Fan Wang, Qing-Pi Yan. Dual RNA-seq provides novel insight into the roles of dksA from Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea). Zoological Research, 2020, 41(4): 410-422. doi: 10.24272/j.issn.2095-8137.2020.048

Dual RNA-seq provides novel insight into the roles of dksA from Pseudomonas plecoglossicida in pathogen-host interactions with large yellow croakers (Larimichthys crocea)

doi: 10.24272/j.issn.2095-8137.2020.048
Funds:  This work was supported by the National Natural Science Foundation of China (31672694), Fujian Provincial Special Fund for Marine and Fishery Protection and Development (MCZ[2019]062), and Open Fund of Fujian Province Key Laboratory of Special Aquatic Formula Feed (2019-01)
More Information
  • Corresponding author: E-mail: yanqp@jmu.edu.cn
  • Received Date: 2020-02-20
  • Accepted Date: 2020-04-21
  • Available Online: 2020-05-13
  • Publish Date: 2020-07-18
  • Pseudomonas plecoglossicida is a rod-shaped, gram-negative bacterium with flagella. It causes visceral white spot disease and high mortality in Larimichthys crocea during culture, resulting in serious economic loss. Analysis of transcriptome and quantitative real-time polymerase chain reaction (PCR) data showed that dksA gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides (log2FC=3.12, P<0.001). RNAi of five shRNAs significantly reduced the expression of dksA in P. plecoglossicida, and the optimal silencing efficiency was 96.23%. Compared with wild-type strains, the symptoms of visceral white spot disease in L. crocea infected with RNAi strains were reduced, with time of death delayed by 48 h and mortality reduced by 25%. The dksA silencing led to a substantial down-regulation in cellular component-, flagellum-, and ribosome assembly-related genes in P. plecoglossicida, and the significant up-regulation of fliC may be a way in which virulence is maintained in P. plecoglossicida. The GO and KEGG results showed that RNAi strain infection in L. crocea led to the down-regulation of inflammatory factor genes in immune-related pathways, which were associated with multiple immune response processes. Results also showed that dksA was a virulence gene in P. plecoglossicida. Compared with the wild-type strains, RNAi strain infection induced a weaker immune response in L. crocea.

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