Volume 41 Issue 4
Jul.  2020
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Zhong-Yuan Shen, Dan Yu, Xin Gao, Fu-Tie Zhang, Huan-Zhang Liu. Genetic diversity and reproductive success of a wild population of Chinese sturgeon (Acipenser sinensis) from the Yangtze River inferred from juveniles born in 2014. Zoological Research, 2020, 41(4): 423-430. doi: 10.24272/j.issn.2095-8137.2020.011
Citation: Zhong-Yuan Shen, Dan Yu, Xin Gao, Fu-Tie Zhang, Huan-Zhang Liu. Genetic diversity and reproductive success of a wild population of Chinese sturgeon (Acipenser sinensis) from the Yangtze River inferred from juveniles born in 2014. Zoological Research, 2020, 41(4): 423-430. doi: 10.24272/j.issn.2095-8137.2020.011

Genetic diversity and reproductive success of a wild population of Chinese sturgeon (Acipenser sinensis) from the Yangtze River inferred from juveniles born in 2014

doi: 10.24272/j.issn.2095-8137.2020.011
Funds:  This study was funded by the National Key R & D Program of China (2018YFD0900801); Strategic Priority Research Program of Chinese Academy of Sciences (XDB31040000); Follow-Up Work of the Three Gorges Project (2136902); and Sino BON-Inland Water Fish Diversity Observation Network
More Information
  • Corresponding author: E-mail: hzliu@ihb.ac.cn
  • Received Date: 2019-12-20
  • Accepted Date: 2020-05-06
  • Available Online: 2020-06-02
  • Publish Date: 2020-07-18
  • The Chinese sturgeon (Acipenser sinensis Gray, 1835) is a large anadromous fish species, which is under considerable threat due to dramatic declines in population numbers. In the current study, population genetic diversity and individual reproductive success were assessed using nuclear microsatellite markers (simple sequence repeat, SSR) and complete mitochondrial (mtDNA) genome analysis of juveniles born in 2014. Results showed the existence of size polymorphism in the mtDNA genome of Chinese sturgeon, which was caused by a repeat motif. Population genetic diversity was high based on both SSR (Ho: 0.728±0.211; He: 0.779±0.122) and mtDNA genome analyses (H: 0.876±0.0035; Pi: 0.0011±0.0010). A positive inbreeding coefficient (FIS: 0.066±0.143) was also found, indicating the occurrence of inbreeding. Reconstruction of sibling groups identified 11 mothers and 11 fathers involved in reproduction of Chinese sturgeons in 2014. Variance in individual reproductive success was not significant, with reproductive success of parent fish instead shown to be relatively even (P=0.997>0.05), thus suggesting the absence of sweepstakes reproductive success (SRS). These results indicate that, in regard to conservation, loss of genetic diversity due to the effects of SRS is not of particular concern. However, we must focus on having an adequate number of adults and suitable environmental conditions to ensure that fish can reproduce.

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  • [1]
    Aljanabi SM, Martinez I. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25(22): 4692−4693. doi:  10.1093/nar/25.22.4692
    [2]
    Bentzen P, Brown GC, Leggett WC. 1989. Mitochondrial DNA polymorphism, population structure, and life history variation in American shad (Alosa sapidissima). Canadian Journal of Fisheries and Aquatic Sciences, 46(8): 1446−1454. doi:  10.1139/f89-184
    [3]
    Bermingham E, Lamb T, Avise JC. 1986. Size polymorphism and heteropiasmy in the mitochondrial DNA of lower vertebrates. Journal of Heredity, 77(4): 249−252. doi:  10.1093/oxfordjournals.jhered.a110230
    [4]
    Brook BW, Tonkyn DW, O’Grady JJ, Frankham R. 2002. Contribution of inbreeding to extinction risk in threatened species. Conservation Ecology, 6(1): 16. doi:  10.5751/ES-00387-060116
    [5]
    Brown JR, Beckenbach AT, Smith MJ. 1992. Mitochondrial DNA length variation and Heteroplasmy in populations of white sturgeon (Acipenser transmontanus). Genetics, 132(1): 221−228.
    [6]
    Burland T G. 2001. DNASTAR's Lasergene sequence analysis software. Methods in Molecular Biology, 132: 71−79.
    [7]
    Chang T, Lin PC, Gao X, Liu F, Duan ZH, Liu HZ. 2017. Using adaptive resolution imaging sonar to investigate Chinese sturgeon (Acipenser sinensis Gray, 1835) behaviour on its only spawning ground in the Yangtze River. Journal of Applied Ichthyology, 33(4): 681−688. doi:  10.1111/jai.13406
    [8]
    Christie MR, Marine ML, French RA, Blouin MS. 2012. Genetic adaptation to captivity can occur in a single generation. Proceedings of the National Academy of Sciences of the United States of America, 109(1): 238−242. doi:  10.1073/pnas.1111073109
    [9]
    da Paz Aguiar J, Gomes PFF, Hamoy IG, dos Santos SEB, Schneider H, Sampaio I. 2018. Loss of genetic variability in the captive stocks of tambaqui, Colossoma macropomum (Cuvier, 1818), at breeding centres in Brazil, and their divergence from wild populations. Aquaculture Research, 49(5): 1914−1925. doi:  10.1111/are.13647
    [10]
    Dai ZJ, Liu JT, Wei Q, Chen JY. 2014. Detection of the three gorges dam influence on the Changjiang (Yangtze River) submerged delta. Scientific Reports, 4: 6600.
    [11]
    de Sá Teles Oliveira C, Moreira RFC, Filho AAS, Fonteles SBA, Evangelista-Barreto NS. 2019. Genetic diversity in natural populations of Colossomamacropomum in the Brazilian Amazon region and in populations farmed in Northeast Brazil based on ISSR markers. Aquaculture International, 27(5): 1423−1434. doi:  10.1007/s10499-019-00395-1
    [12]
    Fopp-Bayat D. 2010. Microsatellite DNA variation in the Siberian sturgeon, Acipenser baeri (Actinopterygii, Acipenseriformes, Acipenseridae), Cultured in a Polish fish Farm. Acta Ichthyologica et Piscatoria, 40(1): 21−25. doi:  10.3750/AIP2010.40.1.03
    [13]
    Frankham R. 1995. Effective population size/adult population size ratios in wildlife: a review. Genetics Research, 66(2): 95−107. doi:  10.1017/S0016672300034455
    [14]
    Galtier N, Gouy M, Gautier C. 1996. SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Bioinformatics, 12(6): 543−548. doi:  10.1093/bioinformatics/12.6.543
    [15]
    Gao X, Brosse S, Chen YB, Lek S, Chang JB. 2009. Effects of damming on population sustainability of Chinese sturgeon, Acipenser sinensis: evaluation of optimal conservation measures. Environmental Biology of Fishes, 86(2): 325−336. doi:  10.1007/s10641-009-9521-4
    [16]
    Gao X, Lin PC, Li MZ, Duan ZH, Liu HZ. 2016. Impact of the Three Gorges Dam on the spawning stock and natural reproduction of Chinese sturgeon in Changjiang River, China. Chinese Journal of Oceanology and Limnology, 34(5): 894−901. doi:  10.1007/s00343-016-5027-z
    [17]
    Garant D, Dodson JJ, Bernatchez L. 2001. Genetic evaluation of mating system and determinants of individual reproductive success in Atlantic Salmon (Salmo salar L.). Journal of Heredity, 92(2): 137−145. doi:  10.1093/jhered/92.2.137
    [18]
    Harrisson KA, Pavlova A, Telonis-Scott M, Sunnucks P. 2014. Using genomics to characterize evolutionary potential for conservation of wild populations. Evolutionary Applications, 7(9): 1008−1025. doi:  10.1111/eva.12149
    [19]
    Hedgecock D. 1994. Does variance in reproductive success limit effective population size of marine organisms?. In: Beaumont A. Genetics and Evolution of Aquatic Organisms. London: Chapman and Hall.
    [20]
    Hedgecock D, Pudovkin AI. 2011. Sweepstakes reproductive success in highly fecund marine fish and shellfish: a review and commentary. Bulletin of Marine Science, 87(4): 971−1002. doi:  10.5343/bms.2010.1051
    [21]
    Hedrick P. 2005. Large variance in reproductive success and the Ne/N ratio. Evolution, 59(7): 1596−1599. doi:  10.1111/j.0014-3820.2005.tb01809.x
    [22]
    Jones AT, Lavery SD, Le Port A, Wang YG, Blower D, Ovenden J. 2019. Sweepstakes reproductive success is absent in a New Zealand snapper (Chrysophrus auratus) population protected from fishing despite “tiny” Ne/N ratios elsewhere. Molecular Ecology, 28(12): 2986−2995. doi:  10.1111/mec.15130
    [23]
    Jones OR, Wang JL. 2010. COLONY: a program for parentage and sibship inference from multilocus genotype data. Molecular Ecology Resources, 10(3): 551−555. doi:  10.1111/j.1755-0998.2009.02787.x
    [24]
    Keller LF, Waller DM. 2002. Inbreeding effects in wild populations. Trends in Ecology & Evolution, 17(5): 230−241.
    [25]
    Kynard B, Wei QW, Ke FE. 1995. Use of ultrasonic telemetry to locate the spawning area of Chinese sturgeon. Chinese Science Bulletin, 40(8): 668−671.
    [26]
    Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23(21): 2947−2948. doi:  10.1093/bioinformatics/btm404
    [27]
    Langella O. 2000. POPULATIONS 1.2: population genetic software, individuals or population distance, phylogenetic trees. http://bioinformatics.org/~tryphon/populations/.
    [28]
    Liao XL, Tian H, Zhu B, Chang JB. 2016. The complete mitochondrial genome of Chinese sturgeon (Acipenser sinensis). Mitochondrial DNA Part A, 27(1): 328−329. doi:  10.3109/19401736.2014.892101
    [29]
    Librado P, Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11): 1451−1452. doi:  10.1093/bioinformatics/btp187
    [30]
    Luikart G, Allendorf FW, Cornuet JM, Sherwin WB. 1998. Distortion of allele frequency distributions provides a test for recent population bottlenecks. Journal of Heredity, 89(3): 238−247. doi:  10.1093/jhered/89.3.238
    [31]
    Maddison DR, Maddison WP. 2011[2016-03-10]. Mesquite: a modular system for evolutionary analysis. Current release version: 3.61. http://mesquiteproject.org.
    [32]
    Mitton JB. 1997. Selection in Natural Populations. Oxford: Oxford University Press.
    [33]
    Monnerot M, Mounolou JC, Solignac M. 1984. Intra-individual length heterogeneity of Rana esculenta mitochondrial DNA. Biology of the Cell, 52(3): 213−218.
    [34]
    Nagy S, Poczai P, Cernák I, Gorji AM, Hegedűs G, Taller J. 2012. PICcalc: an online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics, 50(9–10): 670−672.
    [35]
    Reed DH, Frankham R. 2003. Correlation between fitness and genetic diversity. Conservation Biology, 17(1): 230−237. doi:  10.1046/j.1523-1739.2003.01236.x
    [36]
    Richard A, Dionne M, Wang JL, Bernatchez L. 2013. Does catch and release affect the mating system and individual reproductive success of wild Atlantic salmon (Salmo salar L.)?. Molecular Ecology, 22(1): 187−200. doi:  10.1111/mec.12102
    [37]
    Serbezov D, Bernatchez L, Olsen EM, Vøllestad LA. 2010. Mating patterns and determinants of individual reproductive success in brown trout (Salmo trutta) revealed by parentage analysis of an entire stream living population. Molecular Ecology, 19(15): 3193−3205. doi:  10.1111/j.1365-294X.2010.04744.x
    [38]
    Solignac M, Monnerot M, Mounolou JC. 1983. Mitochondrial DNA heteroplasmy in Drosophila mauritiana. Proceedings of the National Academy of Sciences of the United States of America, 80(22): 6942−6946. doi:  10.1073/pnas.80.22.6942
    [39]
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725−2729. doi:  10.1093/molbev/mst197
    [40]
    Thorstensen M, Bates P, Lepla K, Schreier A. 2019. To breed or not to breed? Maintaining genetic diversity in white sturgeon supplementation programs. Conservation Genetics, 20(5): 997−1007. doi:  10.1007/s10592-019-01190-4
    [41]
    Thrall PH, Young A. 2000. Computer note. AUTOTET: a program for analysis of Autotetraploid genotypic data. Journal of Heredity, 91(4): 348−349. doi:  10.1093/jhered/91.4.348
    [42]
    Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P. 2004. Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4(3): 535−538. doi:  10.1111/j.1471-8286.2004.00684.x
    [43]
    Wang JL. 2004. Sibship reconstruction from genetic data with typing errors. Genetics, 166(4): 1963−1979. doi:  10.1534/genetics.166.4.1963
    [44]
    Waples RS. 2016. Tiny estimates of the Ne/N ratio in marine fishes: are they real?. Journal of Fish Biology, 89(6): 2479−2504. doi:  10.1111/jfb.13143
    [45]
    Waples RS, Grewe PM, Bravington MW, Hillary R, Feutry P. 2018. Robust estimates of a high Ne/N ratio in a top marine predator, southern bluefin tuna. Science Advances, 4(7): eaar7759. doi:  10.1126/sciadv.aar7759
    [46]
    Wei QW, Chen XH, Yang DG, Liu JY, Zhu YJ, Zheng WD. 2005. Variations in spawning stock structure of Acipenser sinensis within 24 years since damming of Gezhouba Dam. Journal of Fishery Sciences of China, 12(4): 452−457. (in Chinese)
    [47]
    Wei QW, Ke FE, Zhang JM, Zhuang P, Luo JD, Zhou RQ, Yang WH. 1997. Biology, fisheries, and conservation of sturgeons and paddlefish in China. Environmental Biology of Fishes, 48(1–4): 241−255.
    [48]
    Wei QW. 2019. Conservation Biology of Chinese Sturgeon (Acipenser sinensis). China: Science Press. (in Chinese)
    [49]
    Willi Y, Van Buskirk J, Hoffmann AA. 2006. Limits to the adaptive potential of small populations. Annual Review of Ecology, Evolution, and Systematics, 37: 433−458. doi:  10.1146/annurev.ecolsys.37.091305.110145
    [50]
    Xin MM. 2015. Genetic Characteristics and Parentage Identification of Chinese Sturgeon (Acipenser sinensis) Based on SSR Markers. Master thesis, Southwest University, Chongqing, China.
    [51]
    Yu ZT, Xu YG, Deng ZL. 1986. Reproductive ecology of Chinese sturgeon (Acipenser sinensis) in the lower reaches of Gezhouba water control project. In: Chinese Ichthyological Scoiety. Anthology of Ichthyology. China: Science Press. (in Chinese)
    [52]
    Zhang SM, Deng H, Wang DQ, Zhang YP, Wu QJ. 1999. Mitochondrial DNA length variation and heteroplasmy in Chinese sturgeon (Acipenser sinensis). Acta Genetica Sinica, 26(5): 489−496. (in Chinese)
    [53]
    Zhang SM, Wang DQ, Zhang YP. 2003. Mitochondrial DNA variation, effective female population size and population history of the endangered Chinese sturgeon, Acipenser sinensis. Conservation Genetics, 4(6): 673−683. doi:  10.1023/B:COGE.0000006107.46111.bc
    [54]
    Zhao N, Qiao Y, Zhu B, Liao X L, Pan L, Chang J B. 2015. Identification ability of tetraploid microsatellite loci in parentage analysis. Journal of Applied Ichthyology, 31(4): 614−619. doi:  10.1111/jai.12698
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