1982 Vol. 3, No. 2
1982, 3(2): 105-110.
CHO-K1 cells were blocked at metaphase by colcemid,UV-irradaited,inchbated in medium 199 containing 3H-TαR and colcemid,and then observed by autoradiography.The authors found no convincing evidences to prove that the excision repair occurs in the DNA of metaphase chromosomes.This result is different from that of Ikushima (1977,Exp.Cell Res.,108,444).The possible causes related to such repair deficiency were suggested as follows:(1) High condensation of the DNA in metaphase chromosomes,(2) low activity of DNA polymerase β (which is known as the main repair polymerase),and (3) disintegration of nuclear envelope during metaphase.
We used rats of different days of age to analyes their liver nonhistone chromosomal proteins UP and NP by high resolution isoelectric focusing-SDS polyacrylamide gel electrophoresis the proteins spot showed regular changes.This paper also dicusses the relatonship between the hormone metablic level and gene regulation at different stages of devlopment.
The effect of the defibrase of Agkistrodon acutus venom on blood coagulation system in rabbits both in vitro and vivo was reported in this paper.Our experiments showed that the defibrase had the same effects on the blood coagulation in vitro as thrombin did.The defibrase could transfer the plasma fibrinogen into fibrin.The later was not cross-linked.The principle was able to degrade the α-chain of the fibrinogen monomers,therefore,the thrombosis could not occur.In vivo,the defibrase remarkably prolonged whole blood coagulation time,one-stage prothrombin time and thrombin time.The plasma fibrinogen level in the blood of rabbits was decreased after injection,but there were no effects on the factor Ⅴ,Ⅶ,Ⅻ and platelet facfor level.The Ac-globulin dissolution time was shortened significantly.The experiment both in vitro and in vivo suggests that the defibrase contained both the properties of the fibrinolytic principle and anticoagulant principle.
In this article,we present the observations of the ova of Taenidae which retain,as seen under scanning electron microscope,the premature ova of the Taeniarhynchus saginatus.An ovum comprises seven layers,which actually interpenetrate and interplay as a complete system.When the ovum becomes mature the capsule breaks,the yolk cells and the vitelline membrane udergo degenerative changes,but the embryophore and the hexocanth embryo grow into completion.Many detail descriptions have been made at home and abroad of the ultrastructure of the mature ova of Taeniidae (Inatomi,S.1962;Ishii,Y.1972;Wang Song-Shan and others 1980).These reports lay particular emphasis on the research of the embryophore s ultrastructure,as the embryophore s tubular system has specieal importance to our understanding of the physiology of the ova.Our researches,we study the ova of Taeniidae at an earlier stage,investigate its basic structure and ultrastructural changes in its development.It may help us further understand this new problem.
1982, 3(2): 165-170.
This paper deals with a number of biological problems of colonizing flight of coptotermes formosanus Shiraki.In a group of Coptotermes formosanus Shiraki,colonizing flight is carried out in differnet times.The winged image emerging time varies,there are even overlap phenomena.The colonizing flight occurs in the end of April or the beginning of May in Guangdong province.The peak stage is in the middle of May.The last stage is in the end of May or June,when colonizing flight occurs,the temperature ranges from 21.7 to 29.4 ℃,more often from 26 to 27 ℃.Relative humidity is between 82 to 99% usually 85 to 90%.The atmosphere is below one atmosphere;Rain is not indispensable during the days when flights are colonized.
1982, 3(2): 201-208.
1982, 3(2): 209-212.
1982, 3(2): 213-214.