WANG Yin, XU Ke, SHEN Guo-guang, DU Xiao-yan, ZHOU Yuan-cong. Solubilization of β-Agkistrodotoxin-Binding Protein From Torpedo Electric Organ and Studies on Its Binding Properties With the Toxin. Zoological Research, 1998, 19(6): 429-433.
Citation:
WANG Yin, XU Ke, SHEN Guo-guang, DU Xiao-yan, ZHOU Yuan-cong. Solubilization of β-Agkistrodotoxin-Binding Protein From Torpedo Electric Organ and Studies on Its Binding Properties With the Toxin. Zoological Research, 1998, 19(6): 429-433.
WANG Yin, XU Ke, SHEN Guo-guang, DU Xiao-yan, ZHOU Yuan-cong. Solubilization of β-Agkistrodotoxin-Binding Protein From Torpedo Electric Organ and Studies on Its Binding Properties With the Toxin. Zoological Research, 1998, 19(6): 429-433.
Citation:
WANG Yin, XU Ke, SHEN Guo-guang, DU Xiao-yan, ZHOU Yuan-cong. Solubilization of β-Agkistrodotoxin-Binding Protein From Torpedo Electric Organ and Studies on Its Binding Properties With the Toxin. Zoological Research, 1998, 19(6): 429-433.
Shanghai Institute of Physiology,the Chinese Academy of Sciences,Shanghai 200031 2.Shanghai Institute of Biochemistry,the Chinese Academy of Sciences,Shanghai 200031
It was infered that β-agkistrodotoxin-binding protein is presumably a novel presynaptic functional protein involved in neurotransmitter release.This paper reported studies on solubilization of β-agkistrodotoxin-binding protein from Torpedo californica electric organ,a tissue rich in synapses.The binding assay of this protein was established using a PEG precipitation method,and its binding properties with [125]I labelled β-AgTX was preliminarily determined.The results showed that the density of β-AgTX-binding sites in this tissue is relatively high (much higher than that of rat brain).B[max] is 1580 fmol/mg protein and K[D] value is 5.5×10[-9] mol/L.Triton X-100 was proved an effective detergent for the extraction experiment with a yield of about 50%.Thus a hopeful beginning for isolation and purification of β-agkistrodotoxin-binding protein was accomplished.