SHEN Guo-Guang, XU Ke. 2000. Purification of β-Bungarotoxin-Binding Protein From Rat Diaphragm Muscle. Zoological Research, 21(3): 177-180.
Citation: SHEN Guo-Guang, XU Ke. 2000. Purification of β-Bungarotoxin-Binding Protein From Rat Diaphragm Muscle. Zoological Research, 21(3): 177-180.

Purification of β-Bungarotoxin-Binding Protein From Rat Diaphragm Muscle

  • β-Bungarotoxin-binding sites exist in the presynaptic membrane of rat phrenic nerve with a relatively high density.The purified β-bungarotoxin-binding protein was obtained from rat diaphragm muscle by a few steps (including homogenization,detergent solubilizaton,ion exchange chromatography,lectin affinity chromatography and β-bungarotoxin affinity chromatography) with a total yield of 3%.The specific binding activity of the purified protein preparations was around 1 nmol/mg protein.The pattern of SDS discontinuous buffer system polyacrylamide gel electrophoresis of the final protein preparation showed that two peptides were co-purified and their molecular weights were 61 and 69 kD,respectively.
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