ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. 2010: Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 31(5): 469-475. DOI: 10.3724/SP.J.1141.2010.05469
Citation: ZHAO Cai-Lian, YANG Qi-Wen, HU Jia-Rui, YE Ding, GONG Wu-Ming, Lü Hai-Ying, XU Z. 2010: Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish. Zoological Research, 31(5): 469-475. DOI: 10.3724/SP.J.1141.2010.05469

Identification of zRAP55,a gene ?preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish

  • In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish .zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages Ⅰ and Ⅱ oocytes, whereas no expression is observed in stages Ⅲ and Ⅳ oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.
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