Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene

Xiu-Feng LI; Chong HAN; Cai-Rong ZHONG; Jun-Qiu XU; Jian-Rong HUANG

doi:10.13918/j.issn.2095-8137.2016.5.307

Volume 37 Issue 5

Sep. 2016

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Xiu-Feng LI, Chong HAN, Cai-Rong ZHONG, Jun-Qiu XU, Jian-Rong HUANG. Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Zoological Research, 2016, 37(5): 307-312. doi: 10.13918/j.issn.2095-8137.2016.5.307

Citation:

Xiu-Feng LI, Chong HAN, Cai-Rong ZHONG, Jun-Qiu XU, Jian-Rong HUANG. Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Zoological Research, 2016, 37(5): 307-312. doi: 10.13918/j.issn.2095-8137.2016.5.307

Citation:

Xiu-Feng LI, Chong HAN, Cai-Rong ZHONG, Jun-Qiu XU, Jian-Rong HUANG. Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Zoological Research, 2016, 37(5): 307-312. doi: 10.13918/j.issn.2095-8137.2016.5.307

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Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene

doi: 10.13918/j.issn.2095-8137.2016.5.307

1.
School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;
2.
Management Bureau of Dongzhaigang Mangrove Natural Reserve, Haikou 571129, China

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Corresponding author: Jian-Rong HUANG
Received Date: 2016-07-15
Rev Recd Date: 2016-09-08
Publish Date: 2016-09-18

Abstract

Abstract

Sphaeroma terebrans, a wood-boring isopoda, is distributed worldwide in tropical and subtropical mangroves. The taxonomy of S. terebrans is usually based on morphological characteristics, with its molecular identification still poorly understood. The number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod are considered as the major morphological characteristics in S. terebrans, which can cause difficulty in regards to accurate identification. In this study, we identified S. terebrans via molecular and morphological data. Furthermore, the validity of the mitochondrial cytochrome c oxidase subunit I (COI) gene as a DNA barcode for the identification of genus Sphaeroma, including species S. terebrans, S. retrolaeve, and S. serratum, was examined. The mitochondrial COI gene sequences of all specimens were sequenced and analysed. The interspecific Kimura 2-parameter distances were higher than intraspecific distances and no intraspecific-interspecific distance overlaps were observed. In addition, genetic distance and nucleotide diversity (π) exhibited no differences within S. terebrans. Our results revealed that the mitochondrial COI gene can serve as a valid DNA barcode for the identification of S. terebrans. Furthermore, the number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod were found to be unreliable taxonomic characteristics for S. terebrans.
- Sphaeroma terebrans,
- DNA barcode,
- COI gene,
- Molecular identification

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References(31)

References

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