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Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids

Yu Wang Shan Gao Yue Zhao Chen Wei-Huang Jun-Jie Shao Wang Ni-Ni Ming Li Guang-Xian Zhou Lei Wang Wen-Jing Shen Jing-Tao Xu Wei-Dong Deng Wen Wang Yu-Lin Chen Yu Jiang

Yu Wang, Shan Gao, Yue Zhao, Chen Wei-Huang, Jun-Jie Shao, Wang Ni-Ni, Ming Li, Guang-Xian Zhou, Lei Wang, Wen-Jing Shen, Jing-Tao Xu, Wei-Dong Deng, Wen Wang, Yu-Lin Chen, Yu Jiang. Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids. Zoological Research, 2019, 40(4): 293-305. doi: 10.24272/j.issn.2095-8137.2019.042
Citation: Yu Wang, Shan Gao, Yue Zhao, Chen Wei-Huang, Jun-Jie Shao, Wang Ni-Ni, Ming Li, Guang-Xian Zhou, Lei Wang, Wen-Jing Shen, Jing-Tao Xu, Wei-Dong Deng, Wen Wang, Yu-Lin Chen, Yu Jiang. Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids. Zoological Research, 2019, 40(4): 293-305. doi: 10.24272/j.issn.2095-8137.2019.042

在杂种动物骡子和犏牛中探究等位基因差异表达和差异剪接

doi: 10.24272/j.issn.2095-8137.2019.042

Allele-specific expression and alternative splicing in horse×donkey and cattle×yak hybrids

Funds: This work was supported by grants from the National Natural Science Foundation of China (31572381), National Thousand Youth Talents Plan of the International Science and Technology Cooperation Project of China (2013DFA31420), and Science and Technology Innovation Capability Promotion Program of the Science and Technology Department of Qinghai Province (2015-ZJ-712)
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    Corresponding author: Yu Jiang
  • 摘要: 基因表达水平和基因选择性剪接的分歧是物种进化的重要驱动力,但其分子机制如今仍不清楚。近缘物种的种间杂交个体为分析等位基因差异表达(ASE)和等位基因差异可变剪接(ASS)提供了合适的模型。通过对近缘物种杂种个体(马×驴(骡子)和牛×牦牛(犏牛))的ASE基因和ASS基因的分析可以揭示近缘物种之间的顺式调控元件的差异,并可以消除环境等反式因子的干扰。在此项研究中,我们提供了包括来自马×驴(骡子)的皮肤、肌肉和脑3种组织的19个转录组数据以及来自牛×牦牛(犏牛)的耳和肝脏组织的10个转录组数据来分析互交杂种个体ASE和ASS基因的详细特征。结果显示,在骡子中4.8%–8.7%的基因以及犏牛中的10.8%–16.7%基因分别表现出等位基因差异表达和等位基因差异可变剪接。值得注意的是,lncRNA和假基因相比蛋白质编码基因更可能表现为等位基因差异表达现象。此外,在骡子中被发现的ASE基因和ASS基因参与肌肉力量的调节,而在犏牛中的则参与高海拔适应的相关通路。总之,我们的研究表明,在近缘物种的杂种动物中探索表示出ASE和ASS的基因对于物种进化研究是可行的。
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  • 收稿日期:  2019-04-01
  • 刊出日期:  2019-07-18

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