Bi-PASA Genetic Marker and Polymorphism of the Porcine STCH Gene

Microsomal stress 70 protein ATPase core (STCH) is a member of the stress 70 protein chaperone family and plays a major role in the immune response and disease resistance of host.Based on the conserved regions of STCH gene sequences from human and mouse,a pair of primers was designed for PCR amplification of porcine STCH gene.A fragment of 445 base pairs product in the exon 5 of pig STCH gene was successfully amplified with the primers.The porcine STCH sequence determined from this fragment showed 87.13% and 80.45% nucleotide identity to the corresponding human and mouse STCH sequences,respectively.A single nucleotide polymorphism (SNP) was found at the position 1 050 of coding sequence in the exon 5 of porcine STCH gene according to the sequencing and alignment of STCH genes from Meishan pigs,Yorkshire pigs and PIC commercial pigs.As no cutting sites of all known restriction enzymes could be found for the SNP,Bi-directional PCR amplification of specific alleles (Bi-PASA) was employed to detect the mutation.According to the porcine STCH Bi-PASA marker,the gene allele frequencies and gene polymorphisms were analyzed in the Chinese pig populations (Meishan pigs,Rongchang pigs,Jinhua pigs) and European populations (Yorkshire pigs,Large white pigs) as well as a commercial line (PIC pigs).The STCH Bi-PASA marker established in this study and polymorphic information obtained from population analysis may be helpful for the further association study between the pig STCH gene mutations and economic traits in pigs.