A Study on The isoenzyme During Embryonic Development of Rana chensinensis

This paper reports the analysis of lactate dehydrogenase (LDH) and Esterase isoenzyme (EST) during Embryonic development of Rana chensinensis and optical density scanning.The results show that there are different type LDH isoenzymes in each stages,LDH1 keeps higher activity during whole developing process,and the relative activities of LDH2,LDH3 and LDH5 are lower.The relative activities of LDH4 gradually increases from Tail bud stage.In addition,LDH1,LDH3,LDH4 subbands specificity in the embryonic development has been discovered.The EST are observed from Mouth open stage.It is clear these ontogenetic changes are reflected the action of the differential genes (Ldh-A & Ldh-B).