Study on Venom of Bungarus fasciatus,The Purification and Biochemical Analysis of Cardiotoxic Principles

Fractionation of venom of Bungarus fasciatus was performed on cellulose phosphate p11 by column chromatography.Peak Ⅷ (cardiotoxic principles) was further isolated on SE-Sephadex G-25 and divided into ⅧA and ⅧB.Purified cardiotoxic principle A and B were obtained by column chromatography of ⅧA and ⅧB on Sephadex G-50.A single band without enzyme activity was observed by polyacrylamide gel electrophoresis.The contents and composition of amino-acid are similar to those tested by Shiau Lin et al.