Karyotype Analysis of The Mouse Ascites Hepatoma

In the present paper,karyotype of the mouse ascites hepatoma is analysed with several banding techniques.The results show that the mouse ascites hepatoma is near-diploid with a modal number of 42-43 (13.0-32.0%) and four or five marker chromosomes.By Ag-As procedure,silver stained NORs (Ag-NORs) are visualized as black spherical bodies on 2-3 chromosomes in the karyotype of the mouse ascites hepatoma.Ag-NORs in this mouse ascites hepatoma cell are usually seen to occur at secondary constrictions on the M1 and M3 chromosomes.