cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli

Using RT-PCR and 3-RACE,DMRT1 gene was partially cloned from the black porgy,Acanthopagrus schlegeli.RT-PCR showed that DMRT1 mRNA was only expressed in the testis.When the transcript levels of DMRT1 were measured in the testis of black porgy before sex change,during sex change,or after sex change,no significant changes in the gene expression were observed.The results show that the transcript levels of DMRT1 gene in the testis of black porgy dont vary according to the changes of physiological status and structure of the testis.