Cloning of cDNA Encoding a C-type Lectin from Snake Trimeresurus jerdonii

Total mRNA was extracted from the venom glands of snakes Trimeresurus jerdonii.The cDNAs encoding lectin were amplified by RT-PCR and cloned into PMD18-T vector.The positive clones encoding lectin were selected and sequenced,and was named TJL.The deduced amino acid sequence contains 135 residues,preceded by a signal peptide of 23 residues,including a galactose-binding site and a Ca2+ binding site.This C-type lectin shows great similarity to ones from Viperidae venoms and less similarity to one from Elapidae venom.