Seung Jae Lee, Jeong-Hoon Kim, Euna Jo, Eunkyung Choi, Jinmu Kim, Seok-Gwan Choi, Sangdeok Chung, Hyun-Woo Kim, Hyun Park. 2021. Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology. Zoological Research, 42(1): 124-129. DOI: 10.24272/j.issn.2095-8137.2020.264
Citation: Seung Jae Lee, Jeong-Hoon Kim, Euna Jo, Eunkyung Choi, Jinmu Kim, Seok-Gwan Choi, Sangdeok Chung, Hyun-Woo Kim, Hyun Park. 2021. Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology. Zoological Research, 42(1): 124-129. DOI: 10.24272/j.issn.2095-8137.2020.264

Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology

  • The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.
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