Ya-Peng ZHAO, Xiao-Fu PAN, Jun-Xing YANG, Xiao-Yong CHEN, Xiao-Ai WANG, Zai-Yun LI. 2013: Use of alizarin red S and alizarin complexone for immersion mar­king Sinocyclocheilus grahami. Zoological Research, 34(5): 499-503. DOI: 10.11813/j.issn.0254-5853.2013.5.0499
Citation: Ya-Peng ZHAO, Xiao-Fu PAN, Jun-Xing YANG, Xiao-Yong CHEN, Xiao-Ai WANG, Zai-Yun LI. 2013: Use of alizarin red S and alizarin complexone for immersion mar­king Sinocyclocheilus grahami. Zoological Research, 34(5): 499-503. DOI: 10.11813/j.issn.0254-5853.2013.5.0499

Use of alizarin red S and alizarin complexone for immersion mar­king Sinocyclocheilus grahami

  • In the present study, we examined the potential uses of Alizarin complexone and Alizarin red S to mark S. grahami larvae and juveniles. Individuals of different ages (6 days, 20 days, 90 days) were immersed in four concentrations of a solution of each chemical (50, 100, 150 and 200 mg/L) for different periods of time (4-36 h). Marked individuals were euthanized 4-27 days after immersion, and the lapillus was removed and used to determine mark quality. After marking with 50-150 mg/L ALC and ARS, violet marks were visible under normal light, and scarlet fluorescent marks were visible under 510-560 nm green light. The clear marks under normal light were recorded, and a 100% survival rate was guaranteed under the following conditions: 6 day old larvae immersed in 50 mg/L ALC or ARS for 8h; 20 day old larvae immersed in 50 mg/L ALC or 100 mg/L ARS for 24h; 90 day old juveniles immersed in 100mg/L ALC or 150 mg/L ARS for 24 h.
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