• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
黄晓燕, 李明利, 徐娟, 高跃东, 王文广, 殷安国, 李晓飞, 孙晓梅, 夏雪山, 代解杰. 2013: 树鼩IL-2全长编码序列的克隆及分子特征分析. 动物学研究, 34(2): 121-126. DOI: 10.3724/SP.J.1141.2013.02121
引用本文: 黄晓燕, 李明利, 徐娟, 高跃东, 王文广, 殷安国, 李晓飞, 孙晓梅, 夏雪山, 代解杰. 2013: 树鼩IL-2全长编码序列的克隆及分子特征分析. 动物学研究, 34(2): 121-126. DOI: 10.3724/SP.J.1141.2013.02121
Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. 2013: Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews. Zoological Research, 34(2): 121-126. DOI: 10.3724/SP.J.1141.2013.02121
Citation: Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. 2013: Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews. Zoological Research, 34(2): 121-126. DOI: 10.3724/SP.J.1141.2013.02121

树鼩IL-2全长编码序列的克隆及分子特征分析

Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews

  • 摘要: 树鼩作为多种人类疾病模型已受到广泛关注, 而免疫因子对于树鼩模型评价至关重要, 但目前对其白细胞介素-2(IL-2)的研究鲜有报道。该实验以经ConA (concanavalin)诱导培养的树鼩淋巴细胞总RNA 为模板, RT-PCR 克隆出465 bp 的树鼩IL-2全长编码序列, 并采用Clustal W 软件分析其序列和分子特征。结果表明树鼩IL-2 cDNA 编码一个由154 个氨基酸组成的蛋白质, 其cDNA 及氨基酸序列与人的同源性分别为93%及80%, 且其整体结构与人IL-2 相似。MEGA5.0 软件构建的进化树表明, 树鼩与人及恒河猴的亲缘关系较近。Pymol 软件对树鼩和人IL-2 氨基酸序列进行的三维结构模建表明, 两者的IL-2分子三维空间结构基本相似, 表面大部分区域所带电荷相同, 但在某些区域差异较大, 且树鼩多出一个糖基化位点, 这些差异对抗体的结合可能存在影响。该研究为今后树鼩IL-2 单克隆抗体的制备及功能研究奠定了基础。

     

    Abstract: While the tree shrew (Tupaia belangeri chinensis) is an excellent animal model for studying the mechanisms of human diseases, but few studies examine interleukin-2 (IL-2), an important immune factor in disease model evaluation. In this study, a 465 bp of the full-length IL-2 cDNA encoding sequence was cloned from the RNA of tree shrew spleen lymphocytes, which were then cultivated and stimulated with ConA (concanavalin). Clustal W 2.0 was used to compare and analyze the sequence and molecular characteristics, and establish the similarity of the overall structure of IL-2 between tree shrews and other mammals. The homology of the IL-2 nucleotide sequence between tree shrews and humans was 93%, and the amino acid homology was 80%. The phylogenetic tree results, derived through the Neighbour-Joining method using MEGA5.0, indicated a close genetic relationship between tree shrews, Homo sapiens, and Macaca mulatta. The three-dimensional structure analysis showed that the surface charges in most regions of tree shrew IL-2 were similar to between tree shrews and humans; however, the N-glycosylation sites and local structures were different, which may affect antibody binding. These results provide a fundamental basis for the future study of IL-2 monoclonal antibody in tree shrews, thereby improving their utility as a model.

     

/

返回文章
返回