三种创伤弧菌免疫原的制备及其对黄姑鱼的免疫保护效果
Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora
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摘要: 从创伤弧菌(Vibrio vulnificus)中提取菌体脂多糖(lipopolysaccaride, LPS)和外膜蛋白(outer membrane protein, OMP)并制备福尔马林灭活全菌苗(FKC), 腹腔注射接种黄姑鱼。分别在注射第0、7、14、21和28天后测定了受免鱼血清中凝集抗体效价、血清溶菌酶活性和血液白细胞吞噬活性, 以及免疫28 d后的相对免疫保护率。结果表明, 3种抗原对黄姑鱼均有较强的免疫原性。免疫后, 免疫组血清凝集抗体效价逐渐增高, 第28天时最高; 溶菌酶活性(LMZ)、白细胞吞噬活性(PP)和吞噬指数(PI)显著升高(P<0.01), 第21天达到峰值, 随后逐渐下降。各组之间比较表明, 受免后7、14、21和28 d, 免疫组黄姑鱼凝集抗体效价、PP、PI和LMZ显著高于对照组(P<0.05), LPS和OMP组凝集抗体效价低于FKC组, LPS和OMP组的相对免疫保护率高于FKC组, 各组间免疫保护率大小顺序为LPS组>OMP组>FKC组>对照组。Abstract: Nibea albiflora was immunized by intraperitoneal injection with either Lipopolysaccharide (LPS) or outer membrane protein (OMP) extracted from Vibrio vulnificus or formalin killed Vibrio vulnificus (FKC). The influence of the three antigens on the immunological function of Nibea albiflora was determined at different time points following the injection by testing the agglutinating antibody titers of the serum, lysozyme activity of the serum, phagocytic activity of the blood and the relative survival percentage. The results showed that the three antigens have higher immunogenicity and antigenicity than the control group(injection with sterile saline). The agglutinating antibody titers of the immune challenged groups increased quickly, and were highest on the day 28. The lysozyme activity and phagocytic activity were raised significantly (P<0.01), reaching their top value on day 21, and then the index gradually reduced. The immunological indexes of three immune groups were higher than the control group (P<0.05). The agglutinating antibody titers of the LPS group or the OMP group were lower than the control group, but the relative survival percentage was adverse when challenged with a Vibrio vulnificus infection. The order of relative survival percentage was group LPS>OMP>FKC>Control.