自发性与膳食诱发性食蟹猴2 型糖尿病其相关基因的表达比较

季芳; 靳丽莎; 曾小明; 张秀娟; 张艳春; 孙云霄; 高丽华; 何红; 饶军华; 刘晓明; 彭白露

doi:10.3724/SP.J.1141.2012.01079

自发性与膳食诱发性食蟹猴2 型糖尿病其相关基因的表达比较

Comparison of gene expression between naturally occurring and diet-induced T2DM in cynomolgus monkeys

摘要

摘要: 采用荧光定量PCR 技术对自发性和膳食诱发性T2DM 食蟹猴外周血白细胞中36 个糖尿病相关基因的表达水平进行分析。在36 个基因中, 糖尿病组的G6PC、CCR2B、CTLA4 等19 个基因的表达量与对照组相比存在显著差异(P<0.05), 且这些基因在诱发组和自发组中的表达模式基本一致。36 个基因中, 诱发组基因的表达量普遍高于自发组, 但大部分基因表达量在两组中差异不显著, 表明诱发性和自发性食蟹猴T2DM 模型均可作为糖尿病研究较理想的动物模型。因此, 通过高能量膳食诱导的食蟹猴糖尿病模型可以替代自发的糖尿病猴, 且基因表达量的变化可为疾病的诊断和治疗提供帮助。

Abstract: To explore pathological alteration of T2DM in cynomolgus monkeys, gene expression profiles of peripheral blood leukocytes from spontaneous and diet-induced T2DM models was analyzed using quantitative real-time PCR. Among 36 T2DM associated genes tested, 19 genes (including G6PC, CCR2B, CTLA4) displayed a similar expression pattern in both spontaneous and diet-induced T2DM models and were significantly up-regulated or down-regulated compared to controls. Interestingly, expression abundance of all up-regulated genes in the diet-induced T2DM was stronger, although not significantly, than spontaneous models, indicating diet-induced T2DM in monkeys should be a reliable research model for changes in gene expression. The characteristic gene expression pattern obtained here may be useful for the clinical diagnosis of T2DM.

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