鲤鱼微卫星突变速率和模式(英文）

Rates and patterns of microsatellite mutations in common carp (Cyprinus carpio L.)

摘要: 利用150个微卫星分子标记在F₁代家系的基因型分析过程中，共有27 600个等位基因从亲本向子代传递，其中在5个微卫星座位上检测到6个突变的等位基因。对突变的等位基因数目进行统计分析后得出：鲤鱼平均每个世代每个微卫星座位的突变速率为2.53×10^-4。在发现突变的5个位点中，经测序发现，突变序列中插入1个以上的重复单元就导致了突变的发生。这些突变表明，鲤鱼的微卫星突变没有遵循严格的渐变突变模型（stepwise mutation model，SMM）。该文关于鲤鱼微卫星突变速率和模式的研究将会对统计鲤鱼有效群体的统计提供有效参数。

Abstract: During genotyping 150 microsatellites in a F₁ family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10^-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.