• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
李乙江, 高跃东, 郭 彦, 陆彩霞, 黄京飞, 夏雪山, 代解杰. 2010: 树鼩CD3ε全长编码序列的克隆及分子特征分析. 动物学研究, 31(5): 483-489. DOI: 10.3724/SP.J.1141.2010.05483
引用本文: 李乙江, 高跃东, 郭 彦, 陆彩霞, 黄京飞, 夏雪山, 代解杰. 2010: 树鼩CD3ε全长编码序列的克隆及分子特征分析. 动物学研究, 31(5): 483-489. DOI: 10.3724/SP.J.1141.2010.05483
LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. 2010: Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 31(5): 483-489. DOI: 10.3724/SP.J.1141.2010.05483
Citation: LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. 2010: Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 31(5): 483-489. DOI: 10.3724/SP.J.1141.2010.05483

树鼩CD3ε全长编码序列的克隆及分子特征分析

Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

  • 摘要: 树鼩可作为人类疾病的良好模型,但缺乏对其免疫功能研究的基本标志和单克隆抗体。该研究应用RT-PCR技术,分别从3只树鼩的肝脏、脾脏和血液中克隆了CD 3ε全长编码序列并用Discovery Studio等软件对其分子特征进行了分析。结果显示,树鼩CD3ε cDNA的开放阅读框全长582 bp,编码193个氨基酸。树鼩与其他哺乳动物的CD3ε蛋白整体结构近似,与人和恒河猴CD3ε的亲缘关系较近,胞内域与跨膜域高度保守,但胞外域出现2个潜在的糖基化位点,表面电荷亦存在差异。该研究为今后制备树鼩CD3单克隆抗体及功能研究奠定初步的基础。

     

    Abstract: The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3ε from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3ε was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3ε protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.

     

/

返回文章
返回