• 中文核心期刊要目总览
  • 中国科技核心期刊
  • 中国科学引文数据库(CSCD)
  • 中国科技论文与引文数据库(CSTPCD)
  • 中国学术期刊文摘数据库(CSAD)
  • 中国学术期刊(网络版)(CNKI)
  • 中文科技期刊数据库
  • 万方数据知识服务平台
  • 中国超星期刊域出版平台
  • 国家科技学术期刊开放平台
  • 荷兰文摘与引文数据库(SCOPUS)
  • 日本科学技术振兴机构数据库(JST)
陈军, 李琪, 孔令锋, 郑小东, 于瑞海. 2010: 基于COI序列的DNA条形码在中国沿海缀锦蛤亚科贝类中的应用分析. 动物学研究, 31(4): 345-352. DOI: 10.3724/SP.J.1141.2010.04345
引用本文: 陈军, 李琪, 孔令锋, 郑小东, 于瑞海. 2010: 基于COI序列的DNA条形码在中国沿海缀锦蛤亚科贝类中的应用分析. 动物学研究, 31(4): 345-352. DOI: 10.3724/SP.J.1141.2010.04345
CHEN Jun, LI Qi, KONG Ling-Feng, ZHENG Xiao-Dong, YU Rui-Hai. 2010: COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) along the Coast of CHINA. Zoological Research, 31(4): 345-352. DOI: 10.3724/SP.J.1141.2010.04345
Citation: CHEN Jun, LI Qi, KONG Ling-Feng, ZHENG Xiao-Dong, YU Rui-Hai. 2010: COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) along the Coast of CHINA. Zoological Research, 31(4): 345-352. DOI: 10.3724/SP.J.1141.2010.04345

基于COI序列的DNA条形码在中国沿海缀锦蛤亚科贝类中的应用分析

COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) along the Coast of CHINA

  • 摘要: 该研究探讨了将COI序列应用于中国沿海缀锦蛤亚科贝类物种鉴定的可行性,获得了该亚科5属11种贝类51个个体的43个单倍型序列。 碱基替换饱和性分析表明,颠换未出现饱和现象,而转换在序列分化达到10%至15%时即到达饱和。单倍型Hap33可能是由杂交引起的,排除此单倍型,种内个体间遗传距离在0%到2.02%之间,平均为0.46%,属内不同种个体间遗传距离在17.21%~32.24%之间,平均为24.96%,存在条形码间隙;11种缀锦蛤亚科贝类在邻接树和贝叶斯树上都独立的单系群。该研究表明,基于COI的DNA条形码技术能够将研究所涉及的约98%的缀锦蛤亚科贝类鉴定到种的水平,因此,利用DNA条形码技术可以对缀锦蛤亚科贝类进行有效地分类鉴定。

     

    Abstract: DNA barcoding has exhibited charming effectiveness in species diagnosis, but some studies suggested the proportion of taxa that cannot be barcode-distinguished was still high. In the present study, the efficiency of the DNA barcoding for delimiting species of subfamily Tapetinae along the coast of China was tested. Fifty one original COI sequences of 11 species in five genera were analyzed. Among these sequences, 43 haplotypes were identified. Saturation plots generated for DNA barcode revealed that transitions became saturated after 10% to 15% sequence divergence. However, transversions were not saturated. Excluding Ruditapes variegata haplotype Hap33 that might be the result of a hybridization event, our finding showed that K2P-distances between conspeci?c sequences varied from 0% to 2.02% (0.46% on average), distances between congeneric sequences were from 17.21% to 32.24% (24.96% on average), and all conspeci?cs clustered together in the phylogentic trees. The proportion of individuals that can be distinguished by DNA barcoding was approximately 98% among 51 individuals analyzed in this study. Thus, the results evidenced that subfamily Tapetidae species can be efficiently identified through the use of DNA barcoding.

     

/

返回文章
返回