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夏王晓, 黎浩榕, 盖景浩, 刘耀武, 李鸿辉, 苏艳华, 王海臻, 郭慧芳, 戴雨轩, 刘耀文, 苟兴春. 2021: 大西洋海刺水母高连接度的基因组组装. 动物学研究, 42(1): 130-134. DOI: 10.24272/j.issn.2095-8137.2020.258
引用本文: 夏王晓, 黎浩榕, 盖景浩, 刘耀武, 李鸿辉, 苏艳华, 王海臻, 郭慧芳, 戴雨轩, 刘耀文, 苟兴春. 2021: 大西洋海刺水母高连接度的基因组组装. 动物学研究, 42(1): 130-134. DOI: 10.24272/j.issn.2095-8137.2020.258
Wang-Xiao Xia, Hao-Rong Li, Jing-Hao Ge, Yao-Wu Liu, Hong-Hui Li, Yan-Hua Su, Hai-Zhen Wang, Hui-Fang Guo, Yu-Xuan Dai, Yao-Wen Liu, Xing-Chun Gou. 2021: High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha. Zoological Research, 42(1): 130-134. DOI: 10.24272/j.issn.2095-8137.2020.258
Citation: Wang-Xiao Xia, Hao-Rong Li, Jing-Hao Ge, Yao-Wu Liu, Hong-Hui Li, Yan-Hua Su, Hai-Zhen Wang, Hui-Fang Guo, Yu-Xuan Dai, Yao-Wen Liu, Xing-Chun Gou. 2021: High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha. Zoological Research, 42(1): 130-134. DOI: 10.24272/j.issn.2095-8137.2020.258

大西洋海刺水母高连接度的基因组组装

High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha

  • 摘要: 大西洋海刺水母(Chrysaora quinquecirrha)具有非常重要的生态价值和进化地位。然而,由于使用有限的测序技术和水母本身复杂的基因组等原因,导致之前组装出的大西洋海刺水母基因组序列片段化严重。因此,在本研究中我们使用当前先进的高通量染色体构象捕获(Hi-C)技术来获得高覆盖度的大西洋海刺水母基因组测序数据。然后,我们将得到的Hi-C数据回帖到前期发表的大西洋海刺水母contig水平的基因组上来提高其序列的连接度。最终,我们成功构建出了一个N50长度为3.83Mb的高连接度的大西洋海刺水母基因组序列(包含了1882个scaffold序列),此基因组版本的N50长度是之前所公布contig水平基因组长度的5.23倍。而且,进一步的多种评估分析结果表明该基因组版本具有较高的连接度和准确性。获取大西洋海刺水母高连接度的基因组序列,不仅为我们利用比较基因组学的手段研究水母的进化提供了基础,而且为我们研究水母的生长发育等基本问题提供了重要的资源。

     

    Abstract: The Atlantic sea nettle (Chrysaora quinquecirrha) has an important evolutionary position due to its high ecological value. However, due to limited sequencing technologies and complex jellyfish genomic sequences, the current C. quinquecirrha genome assembly is highly fragmented. Here, we used the most advanced high-throughput chromosome conformation capture (Hi-C) technology to obtain high-coverage sequencing data of the C. quinquecirrha genome. We then anchored these data to the previously published contig-level assembly to improve the genome. Finally, a high-continuity genome sequence of C. quinquecirrha was successfully assembled, which contained 1 882 scaffolds with a N50 length of 3.83 Mb. The N50 length of the genome assembly was 5.23 times longer than the previously released one, and additional analysis revealed that it had a high degree of genomic continuity and accuracy. Acquisition of the high-continuity genome sequence of C. quinquecirrha not only provides a basis for the study of jellyfish evolution through comparative genomics but also provides an important resource for studies on jellyfish growth and development.

     

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