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兰月, 王娇, 杨巧, 唐瑞祥, 周敏, 雷光伦, 李静, 张亮, 岳碧松, 范振鑫. 2020: 血液转录组分析揭示母乳喂养猕猴婴猴的基因表达特征. 动物学研究, 41(4): 431-436. DOI: 10.24272/j.issn.2095-8137.2020.044
引用本文: 兰月, 王娇, 杨巧, 唐瑞祥, 周敏, 雷光伦, 李静, 张亮, 岳碧松, 范振鑫. 2020: 血液转录组分析揭示母乳喂养猕猴婴猴的基因表达特征. 动物学研究, 41(4): 431-436. DOI: 10.24272/j.issn.2095-8137.2020.044
Yue Lan, Jiao Wang, Qiao Yang, Rui-Xiang Tang, Min Zhou, Guang-Lun Lei, Jing Li, Liang Zhang, Bi-Song Yue, Zhen-Xin Fan. 2020: Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants. Zoological Research, 41(4): 431-436. DOI: 10.24272/j.issn.2095-8137.2020.044
Citation: Yue Lan, Jiao Wang, Qiao Yang, Rui-Xiang Tang, Min Zhou, Guang-Lun Lei, Jing Li, Liang Zhang, Bi-Song Yue, Zhen-Xin Fan. 2020: Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants. Zoological Research, 41(4): 431-436. DOI: 10.24272/j.issn.2095-8137.2020.044

血液转录组分析揭示母乳喂养猕猴婴猴的基因表达特征

Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants

  • 摘要: 在母乳喂养期间,婴儿会经历快速生长发育阶段和显著的生理变化。然而,关于母乳喂养期婴儿的基因表达特征和性别特异性基因表达的研究却很少。在这项研究中,我们对16只母乳喂养的猕猴婴猴和他们各自尚在哺乳期的母猴的共32个血液转录组进行了测序分析。我们鉴定到了218个婴猴与母猴之间的差异表达基因(DEG),其中91个DEG在婴猴组中表达上调,127个DEG在婴猴组中表达下调。通过对婴猴的上调DEG和独有的hub基因的功能富集分析,我们发现它们主要分布在免疫、生长和发育等方面。蛋白质互作分析还发现婴猴中的关键基因主要也是与生长发育和免疫相关。此外,我们还在雌雄婴猴之间鉴定到了23个DEG,其中两个DEG位于X染色体,11个位于Y染色体。TMF1监管核内蛋白1 (Trnp1)在哺乳动物中对大脑皮层发育至关重要,该基因在雌性婴猴中显著表达上调。总的来说,我们的研究为非人灵长类哺乳期婴猴的基因表达特征提供了新的见解,并揭示了这些婴猴之间的性别特异性基因表达。

     

    Abstract: During the breast-feeding period, infants undergo remarkable changes, including rapid physiological and developmental growth. However, little is known about gene expression features and sex-specific gene expression in breast-feeding infants. In this study, we sequenced 32 blood transcriptomes from 16 breast-feeding rhesus macaque (Macaca mulatta) infants and their lactating mothers. We identified 218 differentially expressed genes (DEGs) between infants and mothers, including 91 up-regulated and 127 down-regulated DEGs in the infant group. Functional enrichment analysis of the up-regulated DEGs and unique hub genes in infants showed primary enrichment in immunity, growth, and development. Protein-protein interaction analysis also revealed that genes at key positions in infants were mainly related to development and immunity. However, we only detected 23 DEGs between female and male infants, including three DEGs located on chromosome X and 14 DEGs located on chromosome Y. Of these DEGs, TMF1 regulated nuclear protein 1 (Trnp1), which was highly expressed in female infants, is crucial for controlling the tangential and radial expansion of the cerebral cortex in mammals. Thus, our study provides novel insight into the gene expression features of breast-feeding infants in non-human primates (NHPs) and reveals sex-specific gene expression between these infants.

     

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