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郑宁, 王治忠, 王松伟, 杨方家, 朱续涛, 卢晨, Anne Manyande, 饶小平, 徐富强. 2020: 利用两种携带不同荧光蛋白的逆行感染的腺相关病毒证明小鼠副嗅球的向心投射具有弥散特征. 动物学研究, 41(2): 148-156. DOI: 10.24272/j.issn.2095-8137.2020.020
引用本文: 郑宁, 王治忠, 王松伟, 杨方家, 朱续涛, 卢晨, Anne Manyande, 饶小平, 徐富强. 2020: 利用两种携带不同荧光蛋白的逆行感染的腺相关病毒证明小鼠副嗅球的向心投射具有弥散特征. 动物学研究, 41(2): 148-156. DOI: 10.24272/j.issn.2095-8137.2020.020
Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu. 2020: Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 41(2): 148-156. DOI: 10.24272/j.issn.2095-8137.2020.020
Citation: Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu. 2020: Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 41(2): 148-156. DOI: 10.24272/j.issn.2095-8137.2020.020

利用两种携带不同荧光蛋白的逆行感染的腺相关病毒证明小鼠副嗅球的向心投射具有弥散特征

Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

  • 摘要: 脊椎动物拥有主嗅和副嗅两套嗅觉系统,共同感知和处理挥发性和非挥发性的社会性化学信息,进而调控不同的性行为和社会行为。副嗅球是副嗅系统进行信息传递和处理的第一级中枢,位于主嗅球的背后侧。由于其特殊的解剖位置和缺乏特异性分子细胞标记,我们对副嗅球的内部和外部神经网络了解得非常有限。本文中,我们利用可以在轴突末端吸收并逆行传递和感染神经元胞体的腺相关病毒(retro-rAAV),在小鼠副嗅皮层(终纹床核、皮层杏仁核前侧、中央杏仁核和皮层杏仁核后内侧)注射retro-rAAV,成功实现了对小鼠副嗅球内投射神经元的荧光标记,证明了该方法的有效性。在此基础上,我们利用携带不同荧光蛋白的retro-rAAV分别注射在上述不同的副嗅皮层,发现了副嗅球内投射神经元被共标,证明了小鼠副嗅球内投射神经元向心投射具有弥散的特征(1对多),与主嗅球的投射神经元类似。此外,我们还发现投射到不同皮层的副嗅球投射神经元被感染的数量有所不同,表明单个投射神经元的信息在向心传递过程中,可能会传递到不同皮层内不同数量的皮层神经元。这些结果为我们理解脊椎动物副嗅系统对外周环境中的气味信息的编码和解码机制提供了一定的神经环路基础。

     

    Abstract: The accessory olfactory bulb (AOB), located at the posterior dorsal aspect of the main olfactory bulb (MOB), is the first brain relay of the accessory olfactory system (AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system (MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by single-color labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis (BST), anterior cortical amygdalar area (ACo), medial amygdaloid nucleus (MeA), and posteromedial cortical amygdaloid area (PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.

     

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