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徐德立, 徐萌萌, 王德华. 2019: 温度对布氏田鼠抗氧化防御和天然免疫的影响. 动物学研究, 40(4): 305-316. DOI: 10.24272/j.issn.2095-8137.2019.045
引用本文: 徐德立, 徐萌萌, 王德华. 2019: 温度对布氏田鼠抗氧化防御和天然免疫的影响. 动物学研究, 40(4): 305-316. DOI: 10.24272/j.issn.2095-8137.2019.045
De-Li Xu, Meng-Meng Xu, De-Hua Wang. 2019: Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles. Zoological Research, 40(4): 305-316. DOI: 10.24272/j.issn.2095-8137.2019.045
Citation: De-Li Xu, Meng-Meng Xu, De-Hua Wang. 2019: Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles. Zoological Research, 40(4): 305-316. DOI: 10.24272/j.issn.2095-8137.2019.045

温度对布氏田鼠抗氧化防御和天然免疫的影响

Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles

  • 摘要: 环境温度是影响动物许多生理功能如抗氧化能力和免疫的重要因子,抗氧化防御和免疫能力在一定程度上可以反映动物的生存能力。我们以布氏田鼠(Lasiopodomys brandtii)为研究对象,检验高温或低温会抑制其抗氧化防御和天然免疫能力的假说。将30只雄性布氏田鼠随机分为4°C (n=10)、23°C (n=10)和32 °C 组(n=10),处理27天。结果发现低温显著增加了总能量摄入(GEI)以及肝脏、心脏和肾脏鲜重,但降低了总体脂重和胴体干重。随着温度的降低,反映活性氧(ROS)水平的过氧化氢(H2O2)浓度在肝脏中升高,心脏中降低,而在肾脏、睾丸和小肠中没有变化。不同温度组肝脏、心脏、肾脏、睾丸和小肠中反映脂质过氧化程度的丙二醛(MDA)含量均无显著性差异,暗示着高温和低温并没有造成氧化损伤。除了低温增加睾丸过氧化氢酶(CAT)活性外,所检测的上述5种组织中超氧化物歧化酶(SOD)、CAT和总抗氧化能力(T-AOC)均不受低温或高温的影响。与23 °C组相比,4°C组反映天然免疫的血清杀菌能力几乎受到抑制,而脾脏和白细胞总数不受温度处理的影响。温度处理影响睾酮水平,但不影响皮质酮浓度,并且这两种激素与5种组织中的天然免疫、H2O2和MDA浓度、SOD、CAT和 T-AOC活性均不存在相关性。总之,这些结果表明温度对布氏田鼠的氧化应激、抗氧化酶活性和免疫作用具有组织特异性。抗氧化防御能力的上调或维持可能是布氏田鼠在温度高度可变的环境中生存的重要机制。

     

    Abstract: Ambient temperature is an important factor influencing many physiological processes, including antioxidant defense and immunity. In the present study, we tested the hypothesis that antioxidant defense and immunity are suppressed by high and low temperature treatment in Brandt’s voles (Lasiopodomys brandtii). Thirty male voles were randomly assigned into different temperature groups (4, 23, and 32 °C, n=10 for each group), with the treatment course lasting for 27 d. Results showed that low temperature increased gross energy intake (GEI) and liver, heart, and kidney mass, but decreased body fat mass and dry carcass mass. With the decline in temperature, hydrogen peroxide (H2O2) concentration, which is indicative of reactive oxygen species (ROS) levels, increased in the liver, decreased in the heart, and was unchanged in the kidney, testis, and small intestine. Lipid peroxidation indicated by malonaldehyde (MDA) content in the liver, heart, kidney, testis, and small intestine did not differ among groups, implying that high and low temperature did not cause oxidative damage. Similarly, superoxide dismutase (SOD) and catalase (CAT) activities and total antioxidant capacity (T-AOC) in the five tissues did not respond to low or high temperature, except for elevation of CAT activity in the testis upon cold exposure. Bacteria killing capacity, which is indicative of innate immunity, was nearly suppressed in the 4 °C group in contrast to the 23 °C group, whereas spleen mass and white blood cells were unaffected by temperature treatment. The levels of testosterone, but not corticosterone, were influenced by temperature treatment, though neither were correlated with innate immunity, H2O2 and MDA levels, or SOD, CAT, and T-AOC activity in any detected tissues. Overall, these results showed that temperature had different influences on oxidative stress, antioxidant enzymes, and immunity, which depended on the tissues and parameters tested. Up-regulation or maintenance of antioxidant defense might be an important mechanism for voles to survive highly variable environmental temperatures.

     

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