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Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. 2016: 北平顶猴APOBEC3家族基因克隆和抗HIV-1研究. 动物学研究, 37(4): 246-251. DOI: 10.13918/j.issn.2095-8137.2016.4.246
引用本文: Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. 2016: 北平顶猴APOBEC3家族基因克隆和抗HIV-1研究. 动物学研究, 37(4): 246-251. DOI: 10.13918/j.issn.2095-8137.2016.4.246
Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. 2016: Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina). Zoological Research, 37(4): 246-251. DOI: 10.13918/j.issn.2095-8137.2016.4.246
Citation: Xiao-Liang ZHANG, Jia-Hao SONG, Wei PANG, Yong-Tang ZHENG. 2016: Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina). Zoological Research, 37(4): 246-251. DOI: 10.13918/j.issn.2095-8137.2016.4.246

北平顶猴APOBEC3家族基因克隆和抗HIV-1研究

Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)

  • 摘要: HIV-1只能够感染人与少数的灵长类动物。平顶猴是唯一对HIV-1易感的旧大陆猴,这是因为其宿主限制因子TRIM5ɑ基因中插入了亲环蛋白A基因,TRIMCypA蛋白不能限制HIV-1的复制。尽管平顶猴能够感染HIV-1,但其在平顶猴体内的复制水平受到明显抑制,提示可能与其它宿主限制因子的作用有关。早期研究表明灵长类的APOBEC3具有明显抗HIV-1活性,但北平顶猴APOBEC3抑制HIV-1复制方面的研究未见报道。本研究成功克隆了北平顶猴 APOBEC3A~APOBEC3H基因,序列比对及核酸一致性分析发现北平顶猴的APOBEC3A~APOBEC3H基因蛋白编码区序列与恒河猴与南平顶猴的相应基因的序列的一致性最高达99%。与APOBEC3家族其它成员相比,北平顶猴 APOBEC3G与APOBEC3F的抗HIV-1的活性最强。本研究提示APOBEC3家族中APOBEC3G与APOBEC3F也许是限制HIV-1在平顶猴体内复制的重要宿主限制因子之一,对HIV-1感染的平顶猴模型的优化提供了有参考价值的信息。

     

    Abstract: Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.

     

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