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Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. 2016: 转录组分析研究盐度改变引起的香鱼幼鱼基因表达变化. 动物学研究, 37(3): 126-135. DOI: 10.13918/j.issn.2095-8137.2016.3.126
引用本文: Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. 2016: 转录组分析研究盐度改变引起的香鱼幼鱼基因表达变化. 动物学研究, 37(3): 126-135. DOI: 10.13918/j.issn.2095-8137.2016.3.126
Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. 2016: Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change. Zoological Research, 37(3): 126-135. DOI: 10.13918/j.issn.2095-8137.2016.3.126
Citation: Xin-Jiang LU, Hao ZHANG, Guan-Jun YANG, Ming-Yun LI, Jiong CHEN. 2016: Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change. Zoological Research, 37(3): 126-135. DOI: 10.13918/j.issn.2095-8137.2016.3.126

转录组分析研究盐度改变引起的香鱼幼鱼基因表达变化

Comparative transcriptome analysis on the alteration of gene expression in ayu (Plecoglossus altivelis) larvae associated with salinity change

  • 摘要: 香鱼(Plecoglossus altivelis)是一种东亚地区的洄游性鱼类,幼鱼生活在咸淡水(BW)中,成鱼生活在淡水(FW)中。本研究发现FW适应的香鱼幼鱼和BW适应的香鱼幼鱼相比,生长更为缓慢。然而FW适应抑制生长的机制不明。我们通过转录组测序研究FW适应以后香鱼幼鱼的基因表达变化。研究发现在FW适应组和咸淡水适应组相比,158个基因表达上调,而139个基因表达下调。通过GO基因功能富集分析和KEGG信号通路分析发现差异表达的基因和多种生物功能和过程相关,包括神经内分泌学、渗透压调节、能量代谢和细胞骨架。进一步采用荧光定量PCR验证差异表达基因在FW适应香鱼幼鱼的表达,发现碘化甲腺氨酸脱碘酶(ID)、促阿片-黑素细胞皮质素原(POMC)、甜菜碱半胱氨酸甲基转移酶(BHMT)、果糖二磷酸醛缩酶B(aldolase B)、酪氨酸转氨酸(TAT)和钠钾ATP酶(NKA)在香鱼幼鱼FW适应后表达上调,而b型钠尿肽(BNP)和转凝蛋白在香鱼幼鱼FW适应以后表达下调,这个结果和转录组测序的结果一致。我们的数据表明FW适应可能通过改变内分泌激素、能量代谢和直接的渗透压调控而减缓香鱼幼鱼生长。

     

    Abstract: Ayu (Plecoglossus altivelis) fish, which are an amphidromous species distributed in East Asia, live in brackish water (BW) during their larval stage and in fresh water (FW) during their adult stage. In this study, we found that FW-acclimated ayu larvae exhibited a slower growth ratio compared with that of BW-acclimated larvae. However, the mechanism underlying FW acclimation on growth suppression is poorly known. We employed transcriptome analysis to investigate the differential gene expression of FW acclimation by RNA sequencing. We identified 158 upregulated and 139 downregulated transcripts in FW-acclimated ayu larvae compared with that in BW-acclimated larvae. As determined by Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway mapping, functional annotation of the genes covered diverse biological functions and processes, and included neuroendocrinology, osmotic regulation, energy metabolism, and the cytoskeleton. Transcriptional expression of several differentially expressed genes in response to FW acclimation was further confirmed by real-time quantitative PCR. In accordance with transcriptome analysis, iodothyronine deiodinase (ID), pro-opiomelanocortin (POMC), betaine-homocysteine S-methyltransferase 1(BHMT), fructose-bisphosphate aldolase B (aldolase B), tyrosine aminotransferase (TAT), and Na+-K+ ATPase (NKA) were upregulated after FW acclimation. Furthermore, the mRNA expressions of b-type natriuretic peptide (BNP) and transgelin were downregulated after FW acclimation. Our data indicate that FW acclimation reduced the growth rate of ayu larvae, which might result from the expression alteration of genes related to endocrine hormones, energy metabolism, and direct osmoregulation.

     

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