Volume 24 Issue 3
May  2003
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YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.
Citation: YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.

Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri)

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2003-06-22
  • Tree shrew (Tupaia belangeri) is not infected by HIV-1,and its cells apparently support postentry events in HIV-1 infection.Cyclin T1 binding with HIV-1 Tat is responsible for HIV-1 transcription.To understand whether tree shrew cyclin T1 (tsCycT1) binds with HIV-1 Tat protein,the designed primers and RT-PCR were used to amplify the tsCycT1 cDNA.The clone of full-length tsCycT1 cDNA is of 2 175 bp.The homology of tsCycT1 with that of human CycT1 (hCycT1) is 92.6% at nucleotide level and 94.1% at deduced amino acid level,respectively.The first 272 amino acids have 98.8% of homology with hCycT1,and the residue at position C261 is the cysteine.These results suggest that tsCycT1 can form a high-affinity and zinc-dependent complex binding to HIV-1 Tat and TAR,which may support HIV-1 transcription.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri)

Abstract: Tree shrew (Tupaia belangeri) is not infected by HIV-1,and its cells apparently support postentry events in HIV-1 infection.Cyclin T1 binding with HIV-1 Tat is responsible for HIV-1 transcription.To understand whether tree shrew cyclin T1 (tsCycT1) binds with HIV-1 Tat protein,the designed primers and RT-PCR were used to amplify the tsCycT1 cDNA.The clone of full-length tsCycT1 cDNA is of 2 175 bp.The homology of tsCycT1 with that of human CycT1 (hCycT1) is 92.6% at nucleotide level and 94.1% at deduced amino acid level,respectively.The first 272 amino acids have 98.8% of homology with hCycT1,and the residue at position C261 is the cysteine.These results suggest that tsCycT1 can form a high-affinity and zinc-dependent complex binding to HIV-1 Tat and TAR,which may support HIV-1 transcription.

YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.
Citation: YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.

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