Volume 23 Issue 1
Jan.  2002
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DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi. Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene. Zoological Research, 2002, 23(1): 81-83.
Citation: DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi. Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene. Zoological Research, 2002, 23(1): 81-83.

Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2002-02-22
  • Through adjusting doses of regents,experimental conditions and procedure,we extracted DNA from single rotifer using WizardTM genomic DNA purification kit,and sequenced partial COⅠ sequence of Brachionus calyciflorus using the extracted DNA as a template.The obtained sequence shares high similarity with COⅠ sequence of B.plicatilis,showing that it is really a COⅠ sequence of rotifer species,which verified the reliability of our method.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene

Abstract: Through adjusting doses of regents,experimental conditions and procedure,we extracted DNA from single rotifer using WizardTM genomic DNA purification kit,and sequenced partial COⅠ sequence of Brachionus calyciflorus using the extracted DNA as a template.The obtained sequence shares high similarity with COⅠ sequence of B.plicatilis,showing that it is really a COⅠ sequence of rotifer species,which verified the reliability of our method.

DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi. Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene. Zoological Research, 2002, 23(1): 81-83.
Citation: DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi. Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene. Zoological Research, 2002, 23(1): 81-83.

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