Volume 27 Issue 3
May  2006
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DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.
Citation: DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.

Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells

  • Received Date: 2006-12-03
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-06-22
  • Bovine mammary epithelial cells were isolated and purified by using collagenase digestion of the mammary gland tissue for the primary cell culture, and subsequently using trypsin-selected digestion of the cultured cells for cell purification. Morphological observation revealed that the cultured cells possessed the typical character of epithelial cells. Karyotyping analysis showed the normal chromosome number in cultured cells. The tissue-specific expression of cytokeratin 5 and 8 genes in mammary epithelial cells was identified by immunofluorescent cytochemical staining. The transcription of the betacasein gene was detected by RT-PCR, when the purified cells were induced with insulin, hydrocortisone and prolactin in the culture medium without serum. The above results indicate that the purified cells are mammary epithelial cells, which can transcript beta-casein mRNA in the induction condition.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells

Abstract: Bovine mammary epithelial cells were isolated and purified by using collagenase digestion of the mammary gland tissue for the primary cell culture, and subsequently using trypsin-selected digestion of the cultured cells for cell purification. Morphological observation revealed that the cultured cells possessed the typical character of epithelial cells. Karyotyping analysis showed the normal chromosome number in cultured cells. The tissue-specific expression of cytokeratin 5 and 8 genes in mammary epithelial cells was identified by immunofluorescent cytochemical staining. The transcription of the betacasein gene was detected by RT-PCR, when the purified cells were induced with insulin, hydrocortisone and prolactin in the culture medium without serum. The above results indicate that the purified cells are mammary epithelial cells, which can transcript beta-casein mRNA in the induction condition.

DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.
Citation: DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.

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