Volume 15 Issue 2
Mar.  1994
Turn off MathJax
Article Contents

BEN Kun-long, FENG Shu-lin, CAO Xiao-mei et al.. The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component. Zoological Research, 1994, 15(2): 49-61.
Citation: BEN Kun-long, FENG Shu-lin, CAO Xiao-mei et al.. The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component. Zoological Research, 1994, 15(2): 49-61.

The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1994-05-22
  • In order to determine the biological role of antisperm IgA,especially secretory IgA,in immunological infertility and in the development of an antisperm contraceptive vaccine,a panel of monoclonal IgA (moIgA) antibodies to lactate dehydrogenase C4 (LDH-C4) were prepared by intraintestinal immunization,and their heterogeneous isoforms were demonstrated by Western blotting.Most moIgAs (PA1-PA5) were obtained by the combined use of intraintestinal immunization and fusion of Peyers patch cells for parent cells.In the presence of guinea pig serum complement,mouse sperm were immobilized by moIgA PA1,PA2 and PA4.Mouse sperm were agglutinated by PA4 and PA5 at high concentration.The rates of in vitro fertilization were significantly reduced by 3 of 7 moIgA tested,but moIgA (PA1,PA2 and PA5) had no significant effects on mouse in vivo fertilization by passive immunization.Purified murine bile secretory component was assembled with either purified moIgA or moIgA in ascites in vitro.The effects of moIgA on sperm immobilization,sperm agglutination and in vitro fertilization were not significantly changed following the binding of the moIgA with secretory component.These results provide direct evidence of a positive biological role of anti-LDH-C4 moIgA and secretory IgA in sperm function and in vitro fertilization.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(2190) PDF downloads(1985) Cited by()

Related
Proportional views

The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component

Abstract: In order to determine the biological role of antisperm IgA,especially secretory IgA,in immunological infertility and in the development of an antisperm contraceptive vaccine,a panel of monoclonal IgA (moIgA) antibodies to lactate dehydrogenase C4 (LDH-C4) were prepared by intraintestinal immunization,and their heterogeneous isoforms were demonstrated by Western blotting.Most moIgAs (PA1-PA5) were obtained by the combined use of intraintestinal immunization and fusion of Peyers patch cells for parent cells.In the presence of guinea pig serum complement,mouse sperm were immobilized by moIgA PA1,PA2 and PA4.Mouse sperm were agglutinated by PA4 and PA5 at high concentration.The rates of in vitro fertilization were significantly reduced by 3 of 7 moIgA tested,but moIgA (PA1,PA2 and PA5) had no significant effects on mouse in vivo fertilization by passive immunization.Purified murine bile secretory component was assembled with either purified moIgA or moIgA in ascites in vitro.The effects of moIgA on sperm immobilization,sperm agglutination and in vitro fertilization were not significantly changed following the binding of the moIgA with secretory component.These results provide direct evidence of a positive biological role of anti-LDH-C4 moIgA and secretory IgA in sperm function and in vitro fertilization.

BEN Kun-long, FENG Shu-lin, CAO Xiao-mei et al.. The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component. Zoological Research, 1994, 15(2): 49-61.
Citation: BEN Kun-long, FENG Shu-lin, CAO Xiao-mei et al.. The Biological Function of Antimurine Sperm-Specific Lactate Dehydrogenase C4 Monoclonal IgA Assembled With or Without Secretory Component. Zoological Research, 1994, 15(2): 49-61.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return