Cloning of cDNA Encoding a C-type Lectin from Snake Trimeresurus jerdonii
- Received Date: 1900-01-01
- Rev Recd Date: 1900-01-01
- Publish Date: 2003-08-22
Abstract: Total mRNA was extracted from the venom glands of snakes Trimeresurus jerdonii.The cDNAs encoding lectin were amplified by RT-PCR and cloned into PMD18-T vector.The positive clones encoding lectin were selected and sequenced,and was named TJL.The deduced amino acid sequence contains 135 residues,preceded by a signal peptide of 23 residues,including a galactose-binding site and a Ca2+ binding site.This C-type lectin shows great similarity to ones from Viperidae venoms and less similarity to one from Elapidae venom.
|Citation:||LI Rui, WEI Qin, WANG Wan-yu, XIONG Yu-liang. Cloning of cDNA Encoding a C-type Lectin from Snake Trimeresurus jerdonii. Zoological Research, 2003, 24(4): 293-296.|