Effect of Progesterone on the β-Defensin Expression in Epithelial Cells of Mongolian Sheep Fallopian Tubes
- Received Date: 2009-03-31
- Rev Recd Date: 1900-01-01
- Publish Date: 2009-10-22
Abstract: The epithelial cell of the Mongolian sheep's fallopian tubes were cultured as in vitro model, different concentrations of progesterone (10-6, 10-7, 10-8, 10-9 and 10-10 mol/L) were added in the culture, and then the relative expressions of sheep β-defensin-1 (sBD-1) mRNA were surveyed by real time PCR. The results showed that, compared with the control group, the relative expressions of sBD-1 mRNA added with 10-6 and 10-7 mol/L progesterone were significantly higher (P<0.05), and those of 10-8 and 10-9 mol/L groups were remarkably higher (P<0.01). However, there was no significant difference between 10-10 mol/L group and control. Among groups added with progesterone, the sBD-1 mRNA expressions of 10-8 and 10-9 mol/L groups were markedly higher than that of 10-10 mol/L group (P<0.01), but there was no difference among other added groups. The results indicated that progesterone promoted the relative expression of sBD-1 mRNA in the epithelial cell of the fallopian tubes within the indicated concentrations (10-9-10-6 mol/L). And the relative expression of sBD-1 mRNA was varied with the different concentrations of progesterone. In conclusion, the expression of β-defensin was related to progesterone in female reproductive tract.
|Citation:||TANG Bo, XILIN Gao-wa, FU Ben-dong, DU Chen-guang, CAO Gui-fang. Effect of Progesterone on the β-Defensin Expression in Epithelial Cells of Mongolian Sheep Fallopian Tubes. Zoological Research, 2009, 30(5): 497-502. doi: 10.3724/SP.J.1141.2009.05497|