Volume 34 Issue 3
May  2013
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Ru-Jiang LI, Shu-Dong QIU, Hong TIAN, Sheng-Wei ZHOU. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Zoological Research, 2013, 34(3): 238-243. doi: 10.11813/j.issn.0254-5853.2013.3.0238
Citation: Ru-Jiang LI, Shu-Dong QIU, Hong TIAN, Sheng-Wei ZHOU. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Zoological Research, 2013, 34(3): 238-243. doi: 10.11813/j.issn.0254-5853.2013.3.0238

Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice

doi: 10.11813/j.issn.0254-5853.2013.3.0238
  • Received Date: 2012-12-13
  • Rev Recd Date: 2013-01-14
  • Publish Date: 2013-06-08
  • In a previous study, we observed that multiple low doses of streptozotocin (STZ) can induce spontaneous restoration of normoglycemia in adult mouse diabetes models. In the present study, we sought to identify when spontaneous recovery from diabetes occurs and to disclose the changes in the diabetic data of diabetic mice induced by multiple low doses of streptozotocin (MLD-STZ mice). After inducing type 1 diabetes mellitus, radioimmunoassay, indirect immunofluorescence and hematoxylin-eosin staining were used to determine the diabetic data of MLD-STZ mice. In MLD-STZ mice, the diabetic indicators, including food intake, water consumption, body weight, blood glucose level and serum insulin concentration, developed gradually until week 20. Thereafter, the symptoms of diabetes gradually improved. By the week 36, although body weight and β cell masses remained significantly different between the MLD-STZ mice and the age-matched control animals, food intake, water consumption, blood glucose levels and serum insulin concentrations had all returned to normal levels, and no lymphocytic infiltrations were observed in pancreatic islets. This data demonstrates that MLD-STZ can induce spontaneous recovery from diabetes mellitus in adult mice, suggesting further research into the processes by which normoglycemia is recovered.
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  • [1] Bahr J, Klöting N, Klöting I, Follak N. 2011. Transplantation of adipose tissue protects BB/OK rats from type 1 diabetes development. Transplant Immunology, 24(4): 238-240.
    [2] Bouwens L, Rooman I. 2005. Regulation of pancreatic beta-cell mass. Physiological Reviews, 85(4): 1255-1270.
    [3] Dunn JS, Duffy E, Gilmour MK, Kirkpatrick J, McLetchie NG. 1944. Further observations on the effects of alloxan on the pancreatic islets. The Journal of Physiology, 103(2): 233-243.
    [4] Elsner M, Guldbakke B, Tiedge M, Munday R, Lenzen S. 2000. Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin. Diabetologia, 43(12): 1528-1533.
    [5] Hartmann K, Besch W, Zühlke H. 1989. Spontaneous recovery of streptozotocin diabetes in mice. Experimental and Clinical Endocrinology & Diabetes, 93(2-3): 225-230.
    [6] Karabatas LM, Pastorale C, de Bruno LF, Maschi F, Pivetta OH, Lombardo YB, Chemes H, Basabe JC. 2005. Early manifestations in multiple-low-dose streptozotocin-induced diabetes in mice. Pancreas, 30(4): 318-324.
    [7] Leiter EH, Prochazka M, Coleman DL. 1987. The non-obese diabetic (NOD) mouse. American Journal of Pathology, 128(2): 380-383.
    [8] Li L, Seno M, Yamada H, Kojima I. 2003. Betacellulin improves glucose metabolism by promoting conversion of intraislet precursor cells to β-cells in streptozotocin-treated mice. American Journal of Physiology Endocrinology and Metabolism, 285(3): E577-583.
    [9] Like AA, Rossini AA. 1976. Streptozotocin-induced pancreatic insulitis: new model of diabetes mellitus. Science, 193(4251): 415-417.
    [10] Meier JJ, Bhushan A, Butler AE, Rizza RA, Butler PC. 2005. Sustained beta cell apoptosis in patients with long-standing type 1 diabetes: indirect evidence for islet regeneration?. Diabetologia, 28(11): 2221-2228.
    [11] Song Y, Piao SL, Sheng CH, Chang Y, Jin ZX, Zhang GZ. 2007. Improved investigation of C57BL mice T1DM model induced by STZ. Chinese Journal of Laboratory Diagnosis, 11(1): 36-39. [宋旸, 朴松兰, 盛春华, 常颖, 金正贤, 张桂珍. 2007. STZ 诱导C57BL小鼠T1DM模型优化的研究. 中国实验诊断学, 11(1): 36-39.]
    [12] Su EN, Alder VA, Yu DY, Yu PK, Cringle SJ, Yogesan K. 2000. Continued progression of retinopathy despite spontaneous recovery to normoglycemia in a long-term study of streptozotocin-induced diabetes in rats. Graefe’s Archive for Clinical and Experimental Ophthalmology, 238(2): 163-173.
    [13] Wang RN, Bouwens L, Klöppel PG. 1994. Beta-cell proliferation in normal and streptozotocin-treated newborn rats: site, dynamics and capacity. Diabetologia, 37(11): 1088-1096.
    [14] Wang X, Hai CX. 2011. Study of Animal models of diabetes mellitus. Journal of Toxicology, 25(5): 387-390. [王欣, 海春旭. 2011. 糖尿病动物模型研究进展. 毒理学杂志, 25(5): 387-390.]
    [15] Zhao YY, Huang XY, Chen ZW. 2012. Daintain/AIF-1 (Allograft Inflammatory Factor-1) accelerates type 1 diabetes in NOD mice. Biochemical and Biophysical Research Communications, 427(3):513-517.
    [16] Zipris D, Karnieli E. 2002. A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against diabetes in BioBreeding (BB) rats. Journal of Oncology Practice, 3(3): 76-82.
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Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice

doi: 10.11813/j.issn.0254-5853.2013.3.0238

Abstract: In a previous study, we observed that multiple low doses of streptozotocin (STZ) can induce spontaneous restoration of normoglycemia in adult mouse diabetes models. In the present study, we sought to identify when spontaneous recovery from diabetes occurs and to disclose the changes in the diabetic data of diabetic mice induced by multiple low doses of streptozotocin (MLD-STZ mice). After inducing type 1 diabetes mellitus, radioimmunoassay, indirect immunofluorescence and hematoxylin-eosin staining were used to determine the diabetic data of MLD-STZ mice. In MLD-STZ mice, the diabetic indicators, including food intake, water consumption, body weight, blood glucose level and serum insulin concentration, developed gradually until week 20. Thereafter, the symptoms of diabetes gradually improved. By the week 36, although body weight and β cell masses remained significantly different between the MLD-STZ mice and the age-matched control animals, food intake, water consumption, blood glucose levels and serum insulin concentrations had all returned to normal levels, and no lymphocytic infiltrations were observed in pancreatic islets. This data demonstrates that MLD-STZ can induce spontaneous recovery from diabetes mellitus in adult mice, suggesting further research into the processes by which normoglycemia is recovered.

Ru-Jiang LI, Shu-Dong QIU, Hong TIAN, Sheng-Wei ZHOU. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Zoological Research, 2013, 34(3): 238-243. doi: 10.11813/j.issn.0254-5853.2013.3.0238
Citation: Ru-Jiang LI, Shu-Dong QIU, Hong TIAN, Sheng-Wei ZHOU. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Zoological Research, 2013, 34(3): 238-243. doi: 10.11813/j.issn.0254-5853.2013.3.0238
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