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Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda

Ya-Fei DUAN Ping LIU Ji-Tao LI Jian LI Bao-Quan GAO Ping CHEN

Ya-Fei DUAN, Ping LIU, Ji-Tao LI, Jian LI, Bao-Quan GAO, Ping CHEN. Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda. Zoological Research, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039
Citation: Ya-Fei DUAN, Ping LIU, Ji-Tao LI, Jian LI, Bao-Quan GAO, Ping CHEN. Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda. Zoological Research, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039

脊尾白虾组织蛋白酶L基因的克隆及其表达分析

doi: 10.3724/SP.J.1141.2013.01039
基金项目: 国家高技术研究发展计划课题 (2012AA10A409);国家虾产业技术体系 (CARS-47);公益性行业(农业)科研专项 (201103034);中国水产科学研究院基本科研业务费资助 (2013A0701)
详细信息
  • 中图分类号: Q959.223+.63; Q785; Q786

Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda

  • 摘要: 根据本实验室构建的脊尾白虾(Exopalaemon carinicauda)血细胞全长cDNA文库获得的EST序列,利用RACE技术克隆获得脊尾白虾组织蛋白酶L基因的cDNA全长,命名为EcCatL基因。该序列全长1 136 bp,包括5'非编码区24 bp,开放阅读框960 bp和3'非编码区152 bp,开放阅读框共编码319个氨基酸,预测相对分子量为35.30×103,理论等电点为5.27。同源性分析表明,脊尾白虾组织蛋白酶L EcCatL氨基酸序列与其它甲壳动物高度保守,与变色小长臂虾(Palaemonetes varians)及北极甜虾(Pandalus borealis) CatL的同源性分别为92%和76%。系统进化分析表明,EcCatL基因氨基酸序列与变色小长臂虾的CatL聚为一支。荧光定量PCR分析结果表明,EcCatL基因在血细胞、鳃、肝胰腺、肌肉、卵巢、肠、胃及眼柄中均有表达,其中肝胰腺中的相对表达量最高。感染鳗弧菌及WSSV后6 h和12 h,脊尾白虾血细胞和肝胰腺中EcCatL的表达量较对照组均极显著增加(P<0.01),且具有明显的时间差异性,表明EcCatL基因在脊尾白虾免疫反应中具有重要作用。
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  • 收稿日期:  2012-10-31
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  • 刊出日期:  2013-02-08

Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda

doi: 10.3724/SP.J.1141.2013.01039
    基金项目:  国家高技术研究发展计划课题 (2012AA10A409);国家虾产业技术体系 (CARS-47);公益性行业(农业)科研专项 (201103034);中国水产科学研究院基本科研业务费资助 (2013A0701)
  • 中图分类号: Q959.223+.63; Q785; Q786

摘要: 根据本实验室构建的脊尾白虾(Exopalaemon carinicauda)血细胞全长cDNA文库获得的EST序列,利用RACE技术克隆获得脊尾白虾组织蛋白酶L基因的cDNA全长,命名为EcCatL基因。该序列全长1 136 bp,包括5'非编码区24 bp,开放阅读框960 bp和3'非编码区152 bp,开放阅读框共编码319个氨基酸,预测相对分子量为35.30×103,理论等电点为5.27。同源性分析表明,脊尾白虾组织蛋白酶L EcCatL氨基酸序列与其它甲壳动物高度保守,与变色小长臂虾(Palaemonetes varians)及北极甜虾(Pandalus borealis) CatL的同源性分别为92%和76%。系统进化分析表明,EcCatL基因氨基酸序列与变色小长臂虾的CatL聚为一支。荧光定量PCR分析结果表明,EcCatL基因在血细胞、鳃、肝胰腺、肌肉、卵巢、肠、胃及眼柄中均有表达,其中肝胰腺中的相对表达量最高。感染鳗弧菌及WSSV后6 h和12 h,脊尾白虾血细胞和肝胰腺中EcCatL的表达量较对照组均极显著增加(P<0.01),且具有明显的时间差异性,表明EcCatL基因在脊尾白虾免疫反应中具有重要作用。

English Abstract

段亚飞, 刘萍, 李吉涛, 李健, 高保全, 陈萍. 脊尾白虾组织蛋白酶L基因的克隆及其表达分析[J]. 动物学研究, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039
引用本文: 段亚飞, 刘萍, 李吉涛, 李健, 高保全, 陈萍. 脊尾白虾组织蛋白酶L基因的克隆及其表达分析[J]. 动物学研究, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039
Ya-Fei DUAN, Ping LIU, Ji-Tao LI, Jian LI, Bao-Quan GAO, Ping CHEN. Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda. Zoological Research, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039
Citation: Ya-Fei DUAN, Ping LIU, Ji-Tao LI, Jian LI, Bao-Quan GAO, Ping CHEN. Cloning and expression analysis of Cathepsin L cDNA of Exopalaemon carinicauda. Zoological Research, 2013, 34(1): 39-46. doi: 10.3724/SP.J.1141.2013.01039
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