留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm

Yu-Ping Cao Qing-Qing Lin Wan-Yun He Jing Wang Meng-Ying Yi Lu-Chao Lv Jun Yang Jian-Hua Liu Jian-Ying Guo

Yu-Ping Cao, Qing-Qing Lin, Wan-Yun He, Jing Wang, Meng-Ying Yi, Lu-Chao Lv, Jun Yang, Jian-Hua Liu, Jian-Ying Guo. Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm. Zoological Research, 2020, 41(5): 569-575. doi: 10.24272/j.issn.2095-8137.2020.131
Citation: Yu-Ping Cao, Qing-Qing Lin, Wan-Yun He, Jing Wang, Meng-Ying Yi, Lu-Chao Lv, Jun Yang, Jian-Hua Liu, Jian-Ying Guo. Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm. Zoological Research, 2020, 41(5): 569-575. doi: 10.24272/j.issn.2095-8137.2020.131

共选择可能导致了中国某鸡场质粒介导的黏菌素耐药基因mcr-1的高流行率

doi: 10.24272/j.issn.2095-8137.2020.131

Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene mcr-1 in a Chinese broiler farm

Funds: This work was supported in part by the National Natural Science Foundation of China (31830099, 31902322), International Science and Technology Cooperation Project of Xinjiang Production and Construction Corps(XPCC) (2019BC004), Guangdong Special Support Program Innovation Team (2019BT02N054), and Innovation Team Project of Guangdong University (2019KCXTD001)
More Information
  • 摘要: 质粒介导的黏菌素耐药基因mcr-1在全球日渐肆虐,引起了全世界的广泛关注。在对中国某养鸡场进行常规监测时,发现在该鸡场分离到的大肠杆菌普遍对黏菌素耐药(69.9%),其中有53株菌(63.9%)携带mcr-1基因。所有携带mcr-1的大肠杆菌均呈现多重耐药表型,并携带其它在人医临床上备受关注的耐药基因。此外,这些mcr-1基因主要位于IncI2型和IncHI2型质粒上。通过接合转移试验,我们发现mcr-1能与其它耐药基因共转移,如通过IncI2型质粒(n=3)和 IncHI2 型质粒(n=4)与blaCTX-M-55blaCTX-M-14floRfosA3耐药基因共同转移。此外,稳定的mcr-1基因环境(mcr-1-pap2)普遍存在于IncI2型质粒上,而另一种mcr-1的基因环境(ISApl1-mcr-1-pap2-ISApl1)则主要存在于IncHI2型质粒上。我们推测携带mcr-1的IncI2和IncHI2型质粒在该养殖场的流行,以及mcr-1与质粒上其它耐药基因的共选择,可能导致了mcr-1在该养殖场的盛行。我们呼吁在动物生产过程中,应该合理使用抗菌药物。
    #Authors contributed equally to this work
  • Figure  1.  PFGE pattern of mcr-1-positive E. coli

    Table  1.   Antibiotic resistance profiles, resistance genes, and genetic backgrounds and locations of mcr-1 in 53 E. coli isolates

    IsolateaResistance profilebOther resistance genecLocation of mcr-1, sizedGenetic context of mcr-1
    XCLC11AMP, CTX, STR, TET, FFC, CL, FOSblaCTX-M-14,blaCTX-M-64, fosA3IncHI2ISApl1-mcr-1-pap2
    XCLC12AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC16AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55IncHI2ISApl1-mcr-1-pap2
    XCLC26AMP, CTX, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2ISApl1-mcr-1-pap2
    XCLC37AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2ISApl1-mcr-1-pap2
    XCLC31AMP, STR, TET, FFC, CL, CIPIncHI2ISApl1-mcr-1-pap2
    XCLC33AMP, CTX, GEN, TET, FFC, CL, FOS, CIPblaCTX-M-14IncHI2ISApl1-mcr-1-pap2
    XCLC4AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC46AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, blaCTX-M-65, fosA3, floRIncHI2, ~244 kbISApl1-mcr-1-pap2
    XCLC52AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC54AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, blaCTX-M-65, fosA3,floRIncHI2, ~244 kbISApl1-mcr-1-pap2
    XCLC58AMP, CTX, AMK, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3, rmtBIncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC69AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, blaCTX-M-82b, fosA3,floRIncHI2, ~244 kbISApl1-mcr-1-pap2
    XCLC74AMP, CTX, STR, FFC, CL, FOS, CIPfosA3IncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC75AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2ISApl1-mcr-1-pap2-ISApl1
    XCLC78AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-15, fosA3IncHI2ISApl1-mcr-1-pap2
    XCLC82AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPfosA3IncHI2, ~210 kbISApl1-mcr-1-pap2
    XCLC89AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3,floRIncHI2, ~244 kbISApl1-mcr-1-pap2
    XCLC28AMP, CTX, AMK, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, blaCTX-M-55, fosA3, rmtBIncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC27AMP, CTX, STR, TET, FFC, CL, FOS, CIPfosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC40AMP, CTX, GEM, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC41AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC44AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC55AMP, CAZ, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, blaCTX-M-65, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2-ISApl1(IncHI2),mcr-1-pap2(IncI2)
    XCLC6AMP, CTX, GEN, NEO, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC73AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC8AMP, CAZ, CTX, FOX, GEN, STR, TET, FFC, CL, FOS, CIPfosA3IncHI2, IncI2ISApl1-mcr-1-pap2(IncHI2), mcr-1-pap2(IncI2)
    XCLC35eAMP, CAZ, CTX, FOX, AMK, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, blaCTX-M-55, fosA3, rmtBIncI2, ~65 kbmcr-1-pap2
    XCLC5AMP, CTX, AMK, GEN, STR, TET, FFC, CL, FOS, CIPrmtBIncI2, ~63 kbmcr-1-pap2
    XCLC76AMP, CAZ, CTX, AMK, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3, rmtBIncI2, ~65 kbmcr-1-pap2
    XCLC13AMP, GEN, STR, TET, FFC, CL, FOS, CIPfosA3IncI2, ~63 kbISApl1-mcr-1-pap2
    XCLC15AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncI2mcr-1-pap2
    XCLC21AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55IncI2, ~63 kbmcr-1-pap2
    XCLC2AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncI2, ~63kbmcr-1-pap2
    XCLC20AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-64IncI2, ~65 kbmcr-1-pap2
    XCLC24AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-65, fosA3IncI2mcr-1-pap2
    XCLC34AMP, STR, TET, FFC, CL, FOS, CIPfosA3IncI2, ~63 kbISApl1-mcr-1-pap2
    XCLC39AMP, CAZ, CTX, NEO, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncI2, ~63 kbmcr-1-pap2
    XCLC42AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-65, fosA3IncI2, ~63 kbmcr-1-pap2
    XCLC45AMP, CTX, TET, FFC, CL, FOS, CIPblaCTX-M-65, fosA3IncI2mcr-1-pap2
    XCLC48AMP, CAZ, CTX, FOX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-24, blaCTX-M-55, fosA3IncI2ISApl1-mcr-1-pap2
    XCLC50AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-24, fosA3IncI2, ~63 kbmcr-1-pap2
    XCLC53AMP, STR, TET, FFC, CL, FOS, CIPfosA3IncI2ISApl1-mcr-1-pap2
    XCLC56AMP, CTX, STR, TET, FFC, CL, CIPblaCTX-M-15IncI2mcr-1-pap2
    XCLC60AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-65, fosA3IncI2mcr-1-pap2
    XCLC64AMP, CTX, GEN, NEO, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncI2mcr-1-pap2
    XCLC65AMP, CAZ, CTX, GEM, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14, fosA3IncI2mcr-1-pap2
    XCLC71AMP, CAZ, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-55, fosA3IncI2mcr-1-pap2
    XCLC80AMP, CTX, STR, TET, FFC, CL, CIPblaCTX-M-65IncI2, ~63 kbmcr-1-pap2
    XCLC81AMP, CTX, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14IncI2mcr-1-pap2
    XCLC83AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPIncI2mcr-1-pap2
    XCLC92AMP, CTX, STR, TET, FFC, CL, FOS, CIPIncI2mcr-1-pap2
    XCLC85AMP, CTX, GEN, STR, TET, FFC, CL, FOS, CIPblaCTX-M-14IncX4mcr-1-pap2
    a: Isolates from which mcr-1 gene was transferred to recipient by conjugation or transformation are underlined.
    b: AMP: Ampicillin; CAZ: Ceftazidime; CTX: Cefotaxime; FOX: Cefoxitin; AMK: Amikacin; GEN: Gentamicin; NEO: Neomycin; STR: Streptomycin; TET: Tetracycline; FFC: Florfenicol; CL: Colistin; FOS: Fosfomycin; CIP: Ciprofloxacin. Resistance phenotypes transferred to recipient by conjugation are underlined.
    c: Genes co-transferred with mcr-1 by conjugation or transformation as determined by PCR are underlined. –: Not available
    d: Replicon type of plasmid carrying mcr-1 in transconjugant/transformant and approximate size of plasmid are underlined.
    e: Transformant was obtained from this isolate.
    下载: 导出CSV
  • [1] Barton BM, Harding GP, Zuccarelli AJ. 1995. A general method for detecting and sizing large plasmids. Analytical Biochemistry, 226(2): 235−240. doi:  10.1006/abio.1995.1220
    [2] Casal J, Mateu E, Mejia W, Martin M. 2007. Factors associated with routine mass antimicrobial usage in fattening pig units in a high pig-density area. Veterinary Research, 38(3): 481−492. doi:  10.1051/vetres:2007010
    [3] Elbediwi M, Li Y, Paudyal N, Pan H, Li XL, Xie SH, Rajkovic A, Feng YJ, Fang WH, Rankin SC, Yue M. 2019. Global burden of colistin-resistant bacteria: mobilized colistin resistance genes study (1980-2018). Microorganisms, 7(10): 461. doi:  10.3390/microorganisms7100461
    [4] Falagas ME, Kastoris AC, Kapaskelis AM, Karageorgopoulos DE. 2010. Fosfomycin for the treatment of multidrug-resistant, including extended-spectrum β-lactamase producing, Enterobacteriaceae infections: a systematic review. The Lancet Infectious Diseases, 10(1): 43−50. doi:  10.1016/S1473-3099(09)70325-1
    [5] Feng CY, Wen PP, Xu H, Chi XH, Li S, Yu X, Lin XM, Wu SQ, Zheng BW. 2019. Emergence and comparative genomics analysis of extended-spectrum-β-lactamase-producing Escherichia coli carrying mcr-1 in Fennec Fox imported from Sudan to China. mSphere, 4(6): e00732-19. doi:  10.1128/mSphere.00732-19
    [6] Gautom RK. 1997. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. Journal of Clinical Microbiology, 35(11): 2977−2980. doi:  10.1128/JCM.35.11.2977-2980.1997
    [7] Huang X, Yu L, Chen X, Zhi C, Yao X, Liu Y, Wu S, Guo Z, Yi L, Zeng Z, Liu JH. 2017. High prevalence of colistin resistance and mcr-1 Gene in Escherichia coli isolated from food animals in China. Frontiers in Microbiology, 8: 562.
    [8] Lei T, Zhang JM, Jiang FF, He M, Zeng HY, Chen MT, Wu S, Wang J, Ding Y, Wu QP. 2019. First detection of the plasmid-mediated colistin resistance gene mcr-1 in virulent Vibrio parahaemolyticus. International Journal of Food Microbiology, 308: 108290. doi:  10.1016/j.ijfoodmicro.2019.108290
    [9] Lentz SA, De Lima-Morales D, Cuppertino VM, Nunes LDS, Da Motta AS, Zavascki AP, Barth AL, Martins AF. 2016. Letter to the editor: Escherichia coli harbouring mcr-1 gene isolated from poultry not exposed to polymyxins in Brazil. Euro Surveillance, 21(26): 30267. doi:  10.2807/1560-7917.ES.2016.21.26.30267
    [10] Li RC, Xie MM, Zhang JF, Yang ZQ, Liu LZ, Liu XB, Zheng ZW, Chan EWC, Chen S. 2017. Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant. Journal of Antimicrobial Chemotherapy, 72(2): 393−401. doi:  10.1093/jac/dkw411
    [11] Li ZK, Cao YP, Yi LX, Liu JH, Yang QW. 2019. Emergent polymyxin resistance: end of an Era?. Open Forum Infectious Diseases, 6(10): ofz368. doi:  10.1093/ofid/ofz368
    [12] Ling ZR, Yin WJ, Shen ZQ, Wang Y, Shen JZ, Walsh TR. 2020. Epidemiology of mobile colistin resistance genes mcr-1 to mcr-9. Journal of Antimicrobial Chemotherapy. doi:  10.1093/jac/dkaa205.
    [13] Liu LP, He DD, Lv LC, Liu WL, Chen XJ, Zeng ZL, Partridge SR, Liu JH. 2015. blaCTX-M-1/9/1 hybrid genes may have been generated from blaCTX-M-15 on an IncI2 plasmid. Antimicrobial Agents and Chemotherapy, 59(8): 4464−4470. doi:  10.1128/AAC.00501-15
    [14] Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian GB, Dong BL, Huang XH, Yu LF, Gu DX, Ren HW, Chen XJ, Lv LC, He DD, Zhou HW, Liang ZS, Liu JH, Shen JZ. 2016. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. The Lancet Infectious Diseases, 16(2): 161−168. doi:  10.1016/S1473-3099(15)00424-7
    [15] Liu YY, Liu JH. 2018. Monitoring colistin resistance in food animals, an urgent threat. Expert Review of Anti-infective Therapy, 16(6): 443−446. doi:  10.1080/14787210.2018.1481749
    [16] Lv LC, Partridge SR, He LY, Zeng ZL, He DD, Ye JH, Liu JH. 2013. Genetic characterization of IncI2 plasmids carrying blaCTX-M-55 spreading in both pets and food animals in China. Antimicrobial Agents and Chemotherapy, 57(6): 2824−2827. doi:  10.1128/AAC.02155-12
    [17] Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. 2012. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3): 268−281. doi:  10.1111/j.1469-0691.2011.03570.x
    [18] Migura-Garcia L, González-López JJ, Martinez-Urtaza J, Aguirre Sánchez JR, Moreno-Mingorance A, Perez De Rozas A, Höfle U, Ramiro Y, Gonzalez-Escalona N. 2020. mcr-colistin resistance genes mobilized by IncX4, IncHI2, and IncI2 plasmids in Escherichia coli of pigs and White Stork in Spain. Frontiers in Microbiology, 10: 3072. doi:  10.3389/fmicb.2019.03072
    [19] Moawad AA, Hotzel H, Neubauer H, Ehricht R, Monecke S, Tomaso H, Hafez HM, Roesler U, El-Adawy H. 2018. Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli. Gut Pathogens, 10(1): 39. doi:  10.1186/s13099-018-0266-5
    [20] Nang SC, Li J, Velkov T. 2019. The rise and spread of mcr plasmid-mediated polymyxin resistance. Critical Reviews in Microbiology, 45(2): 131−161. doi:  10.1080/1040841X.2018.1492902
    [21] Nation RL, Li J. 2009. Colistin in the 21st century. Current Opinion in Infectious Diseases, 22(6): 535−543. doi:  10.1097/QCO.0b013e328332e672
    [22] Perreten V, Strauss C, Collaud A, Gerber D. 2016. Colistin resistance gene mcr-1 in avian-pathogenic Escherichia coli in South Africa. Antimicrobial Agents and Chemotherapy, 60(7): 4414−4415. doi:  10.1128/AAC.00548-16
    [23] Perrin-Guyomard A, Bruneau M, Houée P, Deleurme K, Legrandois P, Poirier C, Soumet C, Sanders P. 2016. Prevalence of mcr-1 in commensal Escherichia coli from French livestock, 2007 to 2014. Euro Surveillance, 21(6). doi:  10.2807/1560-7917.ES.2016.21.6.30135
    [24] Quan JJ, Li X, Chen Y, Jiang Y, Zhou ZH, Zhang HC, Sun L, Ruan Z, Feng Y, Akova M, Yu YS. 2017. Prevalence of mcr-1 in Escherichia coli and Klebsiella pneumoniae recovered from bloodstream infections in China: a multicentre longitudinal study. The Lancet Infectious Diseases, 17(4): 400−410. doi:  10.1016/S1473-3099(16)30528-X
    [25] Shen ZQ, Wang Y, Shen YB, Shen JZ, Wu CM. 2016. Early emergence of mcr-1 in Escherichia coli from food-producing animals. The Lancet Infectious Diseases, 16(3): 293. doi:  10.1016/S1473-3099(16)00061-X
    [26] Sun J, Zhang HM, Liu YH, Feng YJ. 2018. Towards understanding MCR-like colistin resistance. Trends in Microbiology, 26(9): 794−808. doi:  10.1016/j.tim.2018.02.006
    [27] Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of Clinical Microbiology, 33(9): 2233−2239. doi:  10.1128/JCM.33.9.2233-2239.1995
    [28] Trung NV, Matamoros S, Carrique-Mas JJ, Nghia NH, Nhung NT, Chieu TTB, Mai HH, Van Rooijen W, Campbell J, Wagenaar JA, Hardon A, Mai NTN, Hieu TQ, Thwaites G, De Jong MD, Schultsz C, Hoa NT. 2017. Zoonotic transmission of mcr-1 colistin resistance gene from small-scale poultry farms, Vietnam. Emerging Infectious Diseases, 23(3): 529−532. doi:  10.3201/eid2303.161553
    [29] Wang CC, Feng Y, Liu LN, Wei L, Kang M, Zong ZY. 2020. Identification of novel mobile colistin resistance gene mcr-10. Emerging Microbes & Infections, 9(1): 508−516.
    [30] Wang J, Ma ZB, Zeng ZL, Yang XW, Huang Y, Liu JH. 2017a. The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes. Zoological Research, 38(2): 55−80. doi:  10.24272/j.issn.2095-8137.2017.003
    [31] Wang Y, Tian GB, Zhang R, Shen YB, Tyrrell JM, Huang X, Zhou HW, Lei L, Li HY, Doi Y, Fang Y, Ren HW, Zhong LL, Shen ZQ, Zeng KJ, Wang SL, Liu JH, Wu CM, Walsh TR, Shen JZ. 2017b. Prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study. The Lancet Infectious Diseases, 17(4): 390−399. doi:  10.1016/S1473-3099(16)30527-8
    [32] Wang Y, Zhang RM, Li JY, Wu ZW, Yin WJ, Schwarz S, Tyrrell JM, Zheng YJ, Wang SL, Shen ZQ, Liu ZH, Liu JY, Lei L, Li M, Zhang QD, Wu CM, Zhang QJ, Wu YN, Walsh TR, Shen JZ. 2017c. Comprehensive resistome analysis reveals the prevalence of NDM and MCR-1 in Chinese poultry production. Nature Microbiology, 2: 16260. doi:  10.1038/nmicrobiol.2016.260
    [33] Wu RJ, Yi LX, Yu LF, Wang J, Liu YY, Chen XJ, Lv LC, Yang J, Liu JH. 2018. Fitness advantage of mcr-1-bearing IncI2 and IncX4 plasmids in Vitro. Frontiers in Microbiology, 9: 331. doi:  10.3389/fmicb.2018.00331
    [34] Yang YQ, Li YX, Song T, Yang YX, Jiang W, Zhang AY, Guo XY, Liu BH, Wang YX, Lei CW, Xiang R, Wang HN. 2017. Colistin resistance gene mcr-1 and its variant in Escherichia coli isolates from chickens in China. Antimicrob Agents Chemother, 61(5): e01204-16. doi:  10.1128/AAC.01204-16
    [35] Zhang JL, Chen L, Wang JW, Yassin AK, Butaye P, Kelly P, Gong JS, Guo WN, Li J, Li M, Yang F, Feng ZX, Jiang P, Song CL, Wang YY, You JF, Yang Y, Price S, Qi KZ, Kang Y, Wang CM. 2018. Molecular detection of colistin resistance genes (mcr-1, mcr-2 and mcr-3) in nasal/oropharyngeal and anal/cloacal swabs from pigs and poultry. Scientific Reports, 8(1): 3705. doi:  10.1038/s41598-018-22084-4
    [36] Zhi CP, Lv LC, Yu LF, Doi Y, Liu JH. 2016. Dissemination of the mcr-1 colistin resistance gene. The Lancet Infectious Diseases, 16(3): 292−293. doi:  10.1016/S1473-3099(16)00063-3
  • [1] Jing Wang, Zhen-Bao Ma, Zhen-Ling Zeng, Xue-Wen Yang, Ying Huang, Jian-Hua Liu.  The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes, Zoological Research. doi: 10.24272/j.issn.2095-8137.2017.003
    [2] Ming GUO, Wen-Zhe HO.  Animal models to study Mycobacterium tuberculosis and HIV co-infection, Zoological Research. doi: 10.11813/j.issn.0254-5853.2014.3.163
    [3] Bai-Yu ZHANG, Si-Man LI, Zheng-Hua GAO, Ji-Hong SHEN.  Protective effects of snake venom antimicrobial peptide OH-CATH on E. coli induced rabbit urinary tract infection models, Zoological Research. doi: 10.3724/SP.J.1141.2013.01027
    [4] yu guo-Yu, XIANG Yang, ZHANG Hong-Yun, JIANG Ping, Lee Wen-Hui, ZHANG Yun, ZHANG.  Expression of Bm-TFF2 mutants in Escherichia coli andtheir cell migration-promoting activity, Zoological Research. doi: 10.3724/SP.J.1141.2011.04379
    [5] Chen Xin-xin, Yu guo-yu, ZHAN Yan, Zhang Yun, Shen Ji-hong, Lee We.  Effects of the Antimicrobial Peptide OH-CATH on Escherichia coli, Zoological Research. doi: 10.3724/SP.J.1141.2009.02171
    [6] DAI Qiang, GU Hai-jun , WANG Yue-zhao.  Theories and Models for Habitat Selection, Zoological Research.
    [7] ZHANG Jin-dong, XIONG Ye, FU Zhi-ping, LI Yu-jie, DAI Qiang, WANG Yue-zhao.  Competitive Strategies of Two Species of Co-occuring Tadpoles(in English), Zoological Research.
    [8] LI Chuang-ju, ZHOU Li, YAO Bo, XIA Wei, LI Zhi, WANG Yang, GUI Jian-fang, *.  Differential Analysis of Expressed Sequence Tags from SMART cDNA Plasmid Libraries of the Orange-spotted Grouper (Epinephelus coioides) at Two Different Gonadal Development Stages, Zoological Research.
    [9] ZHAO Yang, ZHAO Ya-jun , *, LI Bao-ming , *, SHI Zheng-xiang.  The Influence of Density and a Partially Shadowed Layer on the Behaviour and Welfare of Broiler Breeders in Battery Cages During Laying, Zoological Research.
    [10] ZHOU Qi, WANG Wen.  Detecting Natural Selection at the DNA Level, Zoological Research.
    [11] BAO Lei, NIU Cui-juan, MA Rui LI Qing-fen.  Sequences and Phylogeny of Partial Mitochondrial COⅠ Gene from Four Brachionus Species, Zoological Research.
    [12] DONG Yun-wei, NIU Cui-juan, BAO Lei, LI Qing-fen, HUANG Chen-xi.  Method for Extracting DNA from Single Rotifer and Sequencing Partical Mitochondria Cytochrome Oxidase Subunit Ⅰ (COⅠ) Gene, Zoological Research.
    [13] HE Zu-ping, YUAN Hui, FENG Mei-fu.  Toxicity of Penicillic Acid in Broiler Chickens, Zoological Research.
    [14] CHEN Zhong, WANG Ting, HUANG Li-ming, FANG Dai-nan.  Effects of GABA on the Heat Stress Broilers, Zoological Research.
    [15] ZHAO Qi-kun.  Ecological Selection of Primate Social Behavior, Zoological Research.
    [16] LUO Hong, LIU Ci-quan, CAO Huai.  Escherichia coli DNA Pribow and Transcription Initiation Sites A Statistical Study, Zoological Research.
    [17] WANG Guo-jie, HAN Zheng-kang.  Effects of Total Isoflavones of Red Clover on Male Broiler Growth and Serum Testosterone Concentration, Zoological Research.
    [18] HOU Yi-di.  Investigation of Infected State of Balantidium coli in Rhesus Monkey Groupos, Zoological Research.
    [19] QIU Xhe-zhen, QU Xian-ming, WU Ke-zuo, DAI Pei-hua, LI Shi-yun.  The Effect of Cecropins D From Antheraea pernyi on Suspensions of E.coli K12D31, Zoological Research.
    [20] LI Jing-yan, CHEN Yun-he, ZHOU Xing-liang, ZHENG Zi-xiu.  Immunoprotective Activity of Ribosomal Preparation From Poisonous Escherichia coli,the Pathogen of Piglet Dysentery, Zoological Research.
  • ZR-2020-131 Supplementary Materials.pdf
  • 加载中
图(1) / 表(1)
计量
  • 文章访问数:  503
  • HTML全文浏览量:  263
  • PDF下载量:  105
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-05-30
  • 录用日期:  2020-07-21
  • 网络出版日期:  2020-07-31
  • 刊出日期:  2020-09-18

目录

    /

    返回文章
    返回